Ornithine Decarboxylase and Polyamine Biosynthesis in Pancreatic Adaptation

  • Christian Löser
  • Ulrike Cleffmann
  • Frauke Alves
  • Ulrich R. Fölsch
  • Werner Creutzfeldt
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 250)


This study was designed to investigate the role of ornithine decarb-oxylase (ODC) and polyamines in pancreatic adaptation. Cholecystokinin (CCK) is well-known to be a potent trophic stimulus on the pancreas. On the other hand, the oral application of the synthetic trypsin inhibitor camostate results in an extensive release of endogenous CCK in rats, α-difluoromethylornithine (DFMO), an irreversible and specific inhibitor of ODC, was applied simultaneously to elucidate the essential role of polyamines in pancreatic growth. Camostate feeding (200 mg/kg b. wt. orally twice a day) resulted in a rapid elevation of ODC activity already after 2 hours, reaching a maximum after 6 hours (about 200fold above controls) followed by a significant increase in putrescine after 4 hours and sper-midine after 24 hours while spermine remained unchanged. The trophic parameters increased as expected in following timercourse: thymidine kinase (12 hours), DNA polymerase (12 hours), protein (24 hours), pancreatic weight (24 hours) and DNA (5 days). DFMO (2% in drinking water + 3×300 mg/kg b. wt. i. p. during daytime) was not able to prevent but significantly delayed and reduced the camostate-induced increase in ODC and polyamines as well as the trophic parameters. These data indicate an essential role for ODC and polyamines in camostate-induced pancreatic growth and hormonal mediated pancreatic adaptation.


Thymidine Kinase Ornithine Decarboxylase Polyamine Biosynthesis Intestinal Adaptation Pancreatic Enzyme Secretion 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Christian Löser
    • 1
  • Ulrike Cleffmann
    • 1
  • Frauke Alves
    • 1
  • Ulrich R. Fölsch
    • 1
  • Werner Creutzfeldt
    • 1
  1. 1.Div. of Gastroenterology and Endocrinology, Dept. of MedicineGeorg-August-UniversityGöttingenGermany

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