Skip to main content
SpringerLink
Log in
Book cover

Progress in Polyamine Research pp 25–35Cite as

Studies on Ornithine Decarboxylase Antizyme

  • Chapter

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 250))

Abstract

Tissue levels of ornithine decarboxylase (ODC) are under negative feedback regulation by polyamines. Thus, exogenously added polyamines cause a rapid decrease of ODC activity. Recent studies in various laboratories have indicated that the rapid decay of ODC activity caused by polyamines is due both to suppression of ODC synthesis (1–3) and to acceleration of ODC degradation (1, 2, 4) and that a new protein synthesis is needed for the latter effect (2). Antizyme was discovered in 1976 by Canellakis and his collaborators as a unique protein inhibitor of ODC induced by polyamines (5, 6). Since then, antizyme has been shown to be present in various tissues and cells as a free form and/or a complex with ODC (7). Previous studies in this and other laboratories have led to a hypothesis that antizyme not only inhibits ODC activity but also accelerates its degradation in a recycling manner (8–10). The strongest evidence for that hypothesis was the existence of a good correlation between ODC decay rate and antizyme/ODC ratio in HTC cells (9). In the present paper, we report the results of our recent studies that support the above hypothesis. We also present some of our results on the cloning of cDNAs for rat liver antizyme.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. R. Kanamoto, K. Utsunomiya, T. Kameji, and S. Hayashi, Effects of putrescine on synthesis and degradation of ornithine decarboxylase in primary cultured hepatocytes, Eur. J. Biochem., 154: 539 (1986)

    Article  PubMed  CAS  Google Scholar 

  2. E. Hölttä and P. Pohjanpelto, Control of ornithine decarboxylase in Chinese hamster ovary cells by polyamines. Translational inhibition of synthesis and acceleration of degradation of the enzyme by putrescine, spermidine, and spermine, J. Biol. Chem., 261: 9502 (1986)

    PubMed  Google Scholar 

  3. L. Persson, I. Holm., and O. Heby, Translational regulation of ornithine decarboxylase by polyamines, FEBS Lett., 205: 175 (1986)

    Article  PubMed  CAS  Google Scholar 

  4. J. R. Glass and E. W. Gerner, Polyamine-mediated turnover of ornithine decarboxylase in Chinese hamster ovary cells, Biochem. J., 236

    Google Scholar 

  5. W. F. Fong, J. S. Heller, and E. S. Canellakis, The appearance of an ornithine decarboxylase inhibitory protein upon addition of putrescine to cell cultures, Biochim. Biophys. Acta, 428: 456 (1876)

    Article  Google Scholar 

  6. J. S. Heller, W. F. Fong, and E. S. Canellakis, Induction of a protein inhibitor to ornithine decarboxylase by the end products of its reaction, Proc. Natl. Acad. Sci. USA, 73: 1858 (1876)

    Article  Google Scholar 

  7. S. Hayashi and E. S. Canellakis, Ornithine decarboxylase antizymes, in “Ornithine Decarboxylase: Biology, Enzymology, and Molecular Genetics,” S. Hayashi, ed., Pergamon Press, Elmsford, N.Y., (In the press)

    Google Scholar 

  8. Y. Murakami, K. Fujita, T. Kameji, and S. Hayashi, Accumulation of ornithine decarboxylase-antizyme complex in HMOA cells, Biochem. J., 225: 689 (1985)

    PubMed  CAS  Google Scholar 

  9. Y. Murakami and S. Hayashi, Role of antizyme in degradation of ornithine decarboxylase in HTC cells, Biochem. J., 226: 893 (1985)

    PubMed  CAS  Google Scholar 

  10. J. E. Seely and A. E. Pegg, Effect of 1,3-diaminopropane on ornithine decarboxylase enzyme protein in thioacetamide-treated rat liver, Biochem. J., 216: 701 (1983)

    PubMed  CAS  Google Scholar 

  11. L. Persson and E. Rosengren, Inhibition of ornithine decarboxylase activity in mouse kidney by administration of diamines, Acta Chem. Scand., 33: 537 (1979)

    Article  Google Scholar 

  12. J. E. Seely and A. E. Pegg, Changes in mouse kidney ornithine decarboxylase activity are brought about by changes in the amount of enzyme protein as measured by radioimmunoassay, J. Biol. Chem., 258: 2496 (1983)

    PubMed  CAS  Google Scholar 

  13. P. H. Laitinen, R.-L. Huhtinen, O. A. Hietala, and A. E. I. Pajunen, Ornithine decarboxylase activity in brain regulated by a specific macromolecule, the antizyme, J. Neurochem., 44: 1885 (1985)

    Article  PubMed  CAS  Google Scholar 

  14. Y. Murakami, M. Marumo, and S. Hayashi, Ornithine decarboxylase antizyme in kidneys of male and female mice, Biochem. J., (In the press)

    Google Scholar 

  15. K. Fujita, Y. Murakami, and S. Hayashi, A macromolecular inhibitor of the antizyme to ornithine decarboxylase, Biochem. J., 204: 647 (1982)

    PubMed  CAS  Google Scholar 

  16. L. Persson, Putrescine turnover in kidneys of testosterone-treated mice, Biochim. Biophys. Acta,, 674: 204 (1981)

    Article  PubMed  CAS  Google Scholar 

  17. J. E. Seely, H. Pösö, and A. E. Pegg, Effect of androgens on turnover of ornithine decarboxylase in mouse kidney. Studies using labeling of the enzyme by reaction with (14C)alpha-difluoromethylornithine, J. Biol. Chem., 257: 7549 (1982)

    PubMed  CAS  Google Scholar 

  18. V. V. Isomaa, A. E. I. Pajunen, C. W. Bardin, and O. A. Jänne, Ornithine decarboxylase in mouse kidney. Purification, characterization, and radioimmunological determination of the enzyme protein, J. Biol. Chem., 258: 6735 (1983)

    PubMed  CAS  Google Scholar 

  19. S. Henningsson and E. Rosengren, Biosynthesis of histamine and putrescine in mice during post-natal development and its hormone dependence, J. Physiol., 245: 467 (1975)

    PubMed  CAS  Google Scholar 

  20. S. I. Harik, M. D. Hollenberg, and S. H. Snyder, Ornithine decarboxylase turnover slowed by alpha-hydrazinoornithine, Mol. Pharmacol., 10: 41 (1974)

    PubMed  CAS  Google Scholar 

  21. H. Inoue, Y. Kato, M. Takigawa, K. Adachi, and Y. Takeda, Effect of DL-alpha-hydrazino-delta-aminovaleric acid, an inhibitor of ornithine decarboxylase, on polyamine metabolism in isoproterenolstimulated mouse parotid glands, J. Biochem., 77: 879 (1975)

    PubMed  CAS  Google Scholar 

  22. P. P. McCann, C. Tardif, M. C. Duchesne, and P. S. Mamont, Effect of alpha-methylornithine on ornithine decarboxylase activity of rat hepatoma cells in culture, Biochem. Biophys. Res. Commun., 76: 893 (1977)

    Article  PubMed  CAS  Google Scholar 

  23. P. P. McCann, Regulation of ornithine decarboxylase in eukaryotes, in “Polyamines in Biomedical Research,” G. M. Gaugas, ed., John Wiley and Sons, New York (1980)

    Google Scholar 

  24. J. L. A. Mitchell, D. W. Maha, P. P. McCann, and P. Qasba, Dicyclohexylamine effects on HTC cell polyamine content and ornithine decarboxylase activity, Biochim. Biophys. Acta, 840: 309 (1985)

    Article  PubMed  CAS  Google Scholar 

  25. E. Karvonen and H. Pösö, Stabilization of ornithine decarboxylase and N1-spermidine acetyltransferase in rat liver by methylglyoxalbis(guanylhydrazone), Biochim. Biophys. Acta, 791: 239 (1984)

    Article  PubMed  CAS  Google Scholar 

  26. P. Nikula, L. Alhonen-Hongisto, and J. Jänne, Effects of bis(guanylhydrazone)s on the activity and expression of ornithine decarboxylase, Biochem. J., 231: 213 (1985)

    PubMed  CAS  Google Scholar 

  27. L. Persson, S. M. Oredsson, S. Anehus, and O. Heby, Ornithine decarboxylase inhibitors increase the cellular content of the enzyme: Implications for translational regulation, Biochem. Biophys. Res. Commun., 131: 239 (1985)

    Article  PubMed  CAS  Google Scholar 

  28. Y. Murakami, M. Nishiyama, and S. Hayashi, Involvement of antizyme in stabilization of ornithine decarboxylase caused by inhibitors of polyamine synthesis, (Submitted to Eur. J. Biochem.)

    Google Scholar 

  29. A. E. Pegg, Recent advances in the biochemistry of polyamines in eukaryotes, Biochem. J., 234: 249 (1986)

    PubMed  CAS  Google Scholar 

  30. H. Onoue, S. Matsufuji, M. Nishiyama, Y. Murakami, and S. Hayashi, Changes in ornithine decarboxylase and antizyme activities in developing mouse brain, Biochem. J., 250: 797 (1988)

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Nutrition, The Jikei University School of Medicine, Tokyo 105, Japan

    S. Hayashi, Y. Murakami, S. Matsufuji, M. Nishiyama, R. Kanamoto & T. Kameji

Authors
  1. S. Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Murakami
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Matsufuji
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Nishiyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Kanamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Kameji
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institute of Biochemistry of Macromolecules, University of Naples, Naples, Italy

    Vincenzo Zappia

  2. The Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey, Pennsylvania, USA

    Anthony E. Pegg

Rights and permissions

Reprints and permissions

Copyright information

© 1988 Plenum Press, New York

About this chapter

Cite this chapter

Hayashi, S., Murakami, Y., Matsufuji, S., Nishiyama, M., Kanamoto, R., Kameji, T. (1988). Studies on Ornithine Decarboxylase Antizyme. In: Zappia, V., Pegg, A.E. (eds) Progress in Polyamine Research. Advances in Experimental Medicine and Biology, vol 250. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5637-0_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-5637-0_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5639-4

  • Online ISBN: 978-1-4684-5637-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation