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Alterations in Gene Expression in Mouse Hepatocarcinogenesis

  • Tommaso A. Dragani
  • Giacomo Manenti
  • Marina R. M. Sacchi
  • Bruno Colombo
  • Giuseppe Della Porta

Abstract

Alteration in gene expression is one of several molecular changes observed in cancer. The great relevance on cell phenotype of quantitative alterations in the levels of particular mRNAs is well documented. To isolate genes under-expressed in mouse liver tumors compared to normal adult liver, we have screened a normal adult liver cDNA library with RNA probes prepared from a normal adult liver and from a hepatocellular carcinoma. Three different clones showed the common feature to be expressed at relatively high levels in normal adult liver and to be undetectable or expressed at 10–50-fold lower levels, in liver carcinomas and in 14-day-old normal liver. Therefore, these clones should represent genes regulated during liver development. Preliminary nucleotide sequence analysis of a 3′ terminal region of one clone indicated that the clone may be identical to mouse major urinary protein. The characterization of the other two clones is in progress.

Keywords

Liver Tumor Differential Screening Cellular Oncogene Hepatocellular Tumor B6C3 Mouse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AFB1

aflatoxin B1

CD

choline-deficient diet

CDE

choline-deficient diet containing ethionine

DAB

pdimethylaminoazobenzene

DEN

diethylnitrosoamine

HO-DHE

l′-hydroxy-2′,3′-dehydroestragole

3′-Me-DAB

3′-methyl-4-dimethylaminoazobenzene

MUP

major urinary protein

N-HO-AAF

N-hydroxy-2-acetylaminofluorene

PB

Phenobarbital

TPA

12-0-tetradecanoyl phorbol-13-acetate

PDGF

platelet-derived growth factor

VC

vinyl carbamate

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Tommaso A. Dragani
    • 1
  • Giacomo Manenti
    • 1
  • Marina R. M. Sacchi
    • 1
  • Bruno Colombo
    • 1
  • Giuseppe Della Porta
    • 1
  1. 1.Division of Experimental Oncology AIstituto Nazionale TumoriMilanItaly

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