Alterations in Gene Expression in Mouse Hepatocarcinogenesis
Alteration in gene expression is one of several molecular changes observed in cancer. The great relevance on cell phenotype of quantitative alterations in the levels of particular mRNAs is well documented. To isolate genes under-expressed in mouse liver tumors compared to normal adult liver, we have screened a normal adult liver cDNA library with RNA probes prepared from a normal adult liver and from a hepatocellular carcinoma. Three different clones showed the common feature to be expressed at relatively high levels in normal adult liver and to be undetectable or expressed at 10–50-fold lower levels, in liver carcinomas and in 14-day-old normal liver. Therefore, these clones should represent genes regulated during liver development. Preliminary nucleotide sequence analysis of a 3′ terminal region of one clone indicated that the clone may be identical to mouse major urinary protein. The characterization of the other two clones is in progress.
KeywordsLiver Tumor Differential Screening Cellular Oncogene Hepatocellular Tumor B6C3 Mouse
choline-deficient diet containing ethionine
major urinary protein
platelet-derived growth factor
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