Effects on Intrinsic Fluorescence Induced by Active Site Modifications of Rhodanese
Rhodanese is a mitochondrial enzyme of unknown physiological function catalyzing “in vitro” the transport of zero valence sulfur from an appropriate donor like thiosulfate to a nucleophilic acceptor. The enzymic reactions occur via a double-displacement with the intermediacy of a sulfur-loaded form of enzyme where the zero valence sulphur is bound to an active site cysteine (CYS 247) in a persulfide linkage (Westley,1973).
KeywordsLonge Lifetime Iodoacetic Acid Active Site Cysteine Difference Absorption Spectrum Priate Donor
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