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Effects on Intrinsic Fluorescence Induced by Active Site Modifications of Rhodanese

  • Alessandro Finazzi Agrò
  • Nicola Rosato
  • Rodolfo Berni
  • Carlo Cannella
Conference paper

Abstract

Rhodanese is a mitochondrial enzyme of unknown physiological function catalyzing “in vitro” the transport of zero valence sulfur from an appropriate donor like thiosulfate to a nucleophilic acceptor. The enzymic reactions occur via a double-displacement with the intermediacy of a sulfur-loaded form of enzyme where the zero valence sulphur is bound to an active site cysteine (CYS 247) in a persulfide linkage (Westley,1973).

Keywords

Longe Lifetime Iodoacetic Acid Active Site Cysteine Difference Absorption Spectrum Priate Donor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Cannella, C. and Berni, R., 1983, Interaction of Rhodanese with Intermediates of Oxygen Reduction, FEBS Lett., 162:180.PubMedCrossRefGoogle Scholar
  2. Finazzi Agrö, A., Federici, G., Giovagnoli, C., Cannella, C., and Cavallini, D., 1972, Effect of Sulfur Binding on Rhodanese Fluorescence, Eur. J. Biochem., 28:89.PubMedCrossRefGoogle Scholar
  3. Horowitz, P., and Criscimagna, N., 1982, The Specificity of Active-Site Alkylation by Iodoacetic Acid in the Enzyme Thiosulfate Sulfurtransferase, Biochim. Biophys. Acta, 702:173.PubMedCrossRefGoogle Scholar
  4. Westley, J., 1973, Rhodanese, Adv. Enzymol. Relat. Areas Mol. Biol., 39:327.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Alessandro Finazzi Agrò
    • 1
  • Nicola Rosato
    • 1
  • Rodolfo Berni
    • 2
  • Carlo Cannella
    • 2
  1. 1.Department of Experimental Medicine and Biochemical SciencesUniversite of Rome “Tor Vergata” and CNR Center for Molecular BiologyRomeItaly
  2. 2.Institutes of Biochemistry and Molecular BiologyUniversity of ParmaParmaItaly

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