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Fluorescence Anisotropy Experiments on Hog Renal Brush Border Membrane Vesicles

  • M. Ameloot
  • H. De Smedt
  • H. Hendrickx
Conference paper

Abstract

In a temperature study of the solute transport in hog renal brush border membrane (BBM) vesicles under tracer exchange conditions, discontinuities were observed in the Arrhenius plots for sodium-dependent D-glucose and phosphate uptake (De Smedt and Kinne, 1981). The most striking change in the slope of the Arrhenius plots occurred between 12 and 15°C. It has been suggested then that this behavior can be explained by an altered activity of intrinsic membrane proteins due to a change in membrane organization.

Keywords

Tryptophan Residue Brush Border Membrane Fluorescence Decay Fluorescence Anisotropy Brush Border Membrane Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ameloot, M., Hendrickx, H., Herreman, W., Pottel, H., Cauwelaert, F. V., and van der Meer, W., 1984, Biophys. J., 46:525PubMedCrossRefGoogle Scholar
  2. De Smedt, H., and Kinne, R., 1981, Biochim. Biophys. Acta, 648:247PubMedCrossRefGoogle Scholar
  3. van der Meer, W., Pottel, H., Herreman, W., Ameloot, M., Hendrickx, H., and Schröder, H., 1984, Biophys. J., 46:515.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • M. Ameloot
    • 1
  • H. De Smedt
    • 2
  • H. Hendrickx
    • 1
  1. 1.Limburgs Universitair Centrum, BiophysicsDiepenbeekBelgium
  2. 2.Laboratorium voor FysiologieKatholieke Universiteit LeuvenLeuvenBelgium

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