Usefulness of the Linkage Concept to Understanding the Regulation of Rat Liver Phosphofructokinase
Although this tribute to Gregorio Weber has quite appropriately focused on the use of fluorescence methodologies in the study of macro-molecules, most of which he pioneered, it is perhaps also fitting to discuss other contributions to our current thinking regarding protein biophysics, that do not necessarily involve the exploitation of the fluorescence phenomenon, to which the Professor has made seminal contributions as well. I refer specifically to several papers (Weber, 1971, 1972, 1975), written in the early 1970’s, pertaining to protein ligand binding and the ramifications of the principles of linkage that must be satisfied when evaluating binding behavior. After a brief review of the points made in these papers, I would like to then discuss how the concept of linkage, as elaborated by Dr. Weber, has proven to be invaluable in providing a greater insight into the mechanisms governing the allosteric regulation of an enzyme of considerable interest to those studying the metabolic control of hepatic carbohydrate metabolism; namely, rat liver phosphofructokinase (PFK).
KeywordsFree Energy American Chemical Society Fluorescence Polarization Standard Free Energy Rabbit Muscle
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- Aaronson, R. P., and Frieden, C., 1971, Rabbit Muscle Phosphofructokinase: Studies on the Polymerization, J. Biol. Chem., 247:7502.Google Scholar
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