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Digitized Fluorescence Microscopy in Cell Biology Applications

  • Ken Jacobson
  • Akira Ishihara
  • Bruce Holifield
  • James DiGuiseppi
Conference paper

Abstract

Fluorescence microscopy is a widely used technique for studying the location and amount of certain components on or within cells. Specific cellular components can be visualized by linking fluorescent dyes to exogenous macromolecules such as antibodies (immunofluorescence) and ligands, or by producing fluorescent analogs of small molecules normally found within cells. In the past, these approaches have involved fixation of cells and the use of high intensity excitation to image the specimen and produce photographs. More recently, low light level video cameras coupled with frame rate video digitizers have been employed to capture cellular images from the fluorescence microscope. The current and growing appeal of digitized fluorescence microscopy (DFM) in cell biology lies in its ability to quantitatively image the distribution of single classes of macromolecules, molecules and ions in single living cells. In this sense, it is really a new form of optical microscopy for living cells in which the image produced gives directly the distribution of the component probed for. In other forms of live cell light microscopy, we must infer, from other information, the composition of the structure visualized in the image, since we have no a priori knowledge of the molecular structure producing contrast.

Keywords

Isotropic Diffusion Single Living Cell Fluorescent Analog High Intensity Excitation Salivary Gland Nucleus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Ken Jacobson
    • 1
    • 2
  • Akira Ishihara
    • 1
  • Bruce Holifield
    • 1
  • James DiGuiseppi
    • 3
  1. 1.Laboratories for Cell BiologyUniversity of North Carolina at Chapel HillChapel HillUSA
  2. 2.Department of Anatomy and Cancer Research CenterUniversity of North Carolina at Chapel HillChapel HillUSA
  3. 3.School of MedicineUniversity of North Carolina at Chapel HillChapel HillUSA

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