Urokinase:AT-III:PGE1:Methyl Dopa Complex Immobilized Albumin-Blended Chitosan Membranes — Antithrombotic and Permeability Properties
The search for a nonthrombogenic membrane having high permselectivity for hemodialysis applications continues to be a field of extensive investigation. A series of membranes was prepared by air drying the thin layers of albumin:chitosan [a (1→4)-2-amino-2-deoxy-β-D-glucan] blends in various proportions. The albumin blended chitosan membranes showed high permeability properties for low molecular weight compounds. Complexes having fibrinolytic, anticoagulant and antiplatelet activities were prepared by repeated modification of urokinase with antithrombin-III, and methyl dopa:PGE1. A nonthrombogenic albumin:chitosan blended membrane was derived by immobilizing this bioactive complex on them, via the carbodiimide functional moiety. This novel membrane demonstrated good permeability properties for small molecules and showed a dramatic reduction in platelet attachment. Such membranes that have drug complexes immobilized on them may have wider applications in the hemodialysis of patients with hypertension, as well as providing improved permeability and blood compatibility.
KeywordsUric Acid Methyl Dopa Blood Compatibility Permeability Property Chitosan Membrane
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