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Monoclonal Antibodies That Coimmunoprecipitate the 1,4-Dihydropyridine and Phenylalkylamine Receptors and Reveal the Ca2+ Channel Structure

  • Michel Fosset
  • Sylvie Vandaele
  • Jean-Pierre Galizzi
  • Jacques Barhanin
  • Michel Lazdunski
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Monoclonal hybridoma cell lines secreting antibodies against the ( + )PN 200–110 and the ( -)desmethoxyverapamil binding components of the voltage-dependent calcium channel from rabbit transverse-tubule membranes have been isolated. The specificity of these mono clonal antibodies was established by their ability to coimmunoprecipitate (+ )[3 H]PN 200–110 and ( -)[3 H]desmethoxyverapamil receptors. Monoclonal antibodies described in this work cross-reacted with rat, mouse, chicken, and frog skeletal muscle Ca2+ channels, but not with crayfish muscle Ca2+ channels. Cross-reactivity was also detected with membranes prepared from rabbit heart, brain, and intestinal smooth muscle.

Keywords

Subunit Structure Single Polypeptide Chain Binding Component Rabbit Skeletal Muscle Mammalian Skeletal Muscle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Borsotto, M., Barhanin, J., Norman, R. I., and Lazdunski, M., 1984a, Purification of the dihydropyridine receptor of the voltage-dependent Ca2+ channel from skeletal muscle transverse tubules using ( + )[3H]PN 200–110, Biochem. Biophys. Res. Commun. 122:1357–1366.PubMedCrossRefGoogle Scholar
  2. Borsotto, M., Norman, R. I., Fosset, M., and Lazdunski, M., 1984b, Solubilization of the nitrendipine receptor from muscle transverse tubule membranes: Interactions with specific inhibitors of the voltage-dependent Ca2+ channel, Eur. J. Biochem. 142:449–455.PubMedCrossRefGoogle Scholar
  3. Borsotto, M., Barhanin, J., Fosset, M., and Lazdunski, M., 1985, The 1,4-dihydropyridine receptor associated with the skeletal muscle voltage-dependent Ca2+ channel. Purification and subunit composition, J. Biol. Chem. 260:14255–14263.PubMedGoogle Scholar
  4. Casadei, J. M., Gordon, R. D., Lampson, L. A., Schotland, D. L., and Barchi, R. L., 1984, Monoclonal antibodies against the voltage-sensitive Na + channel from mammalian skeletal muscle, Proc. Nat I. Acad. Sci. USA 81:6227–6231.CrossRefGoogle Scholar
  5. Cognard, C., Romey, G., Galizzi, J. P., Fosset, M., and Lazdunski, M., 1986, Dihydropyridinesensitive Ca2+ channels in mammalian skeletal muscle cells in culture: Electrophysiological prop erties and interactions with Ca2+ channel activator (Bay K8644) and inhibitor (PN 200–110), Proc. Naîl. Acad. Sci. USA 83:1518–1522.CrossRefGoogle Scholar
  6. Curtis, B. M., and Catterall, W. A., 1984, Purification of the calcium antagonist receptor of the voltagesensitive calcium channel from skeletal muscle transverse tubules, Biochemistry 23:2113–2118.PubMedCrossRefGoogle Scholar
  7. Curtis, B. M., and Catterall, W. A., 1985, Phosphorylation of the calcium antagonist receptor of the voltage-sensitive calcium channel by cAMP-dependent protein kinase, Proc. Natl. Acad. Sci. USA 82:2528–2532.PubMedCrossRefGoogle Scholar
  8. Ferry, D. R., Rombush, M., Goll, A., and Glossmann, H., 1984, Photoaffinity labeling of Ca2+ channels with [3H]azidopine, FEBS Lett. 169:112–118.PubMedCrossRefGoogle Scholar
  9. Fosset, M., Jaimovich, E., Delpont, E., and Lazdunski, M., 1983, [3H]nitrendipine receptors in skeletal muscle. Properties and preferential localization in transverse tubules, J. Biol. Chem. 258:6086–6092.PubMedGoogle Scholar
  10. Galizzi, J. P., Borsotto, M., Barhanin, J., Fosset, M., and Lazdunski, M., 1986, Characterization and photoaffinity labeling of receptor sites for the Ca2+ channel inhibitors d-cis-diltiazem, (± )bepridil, ( -)desmethoxyverapamil and ( + )PN 200–110 in skeletal muscle transverse tubule membranes, J. Biol. Chem. 261:1393–1397.PubMedGoogle Scholar
  11. Hosey, M. M., Borsotto, M., and Lazdunski, M., 1986, Phosphorylation and dephosphorylation of the major component of the voltage-dependent Ca2+ channel in skeletal muscle membranes by cyclic AMP-and Ca2+-dependent processes, Proc. Natl. Acad. Sci. USA 83:3733–3737.PubMedCrossRefGoogle Scholar
  12. Janis, R. A., and Triggle, D. J., 1984, Calcium Channel Antagonists: New Perspectives from the Radioligand Binding Assay, Vol. 2, Modern Methods Pharmacol. Alan R. Liss, New York, pp. 1–28.Google Scholar
  13. Norman, R. I., Burgess, A. J., Allen, E., and Harrison, T. M., 1987, Monoclonal antibodies against the 1,4-dihydropyridine receptor associated with voltage-sensitive Ca2+ channels, FEBS Lett. 212: 127–132.PubMedCrossRefGoogle Scholar
  14. Schmid, A., Barhanin, J., Coppola, T., Borsotto, M., and Lazdunski, M., 1986, Immunochemical analysis of subunit structure of 1,4-dihydropyridine receptors associated with voltage-dependent Ca2+ channels in skeletal, cardiac and smooth muscles, Biochemistry 25:3492–3495.PubMedCrossRefGoogle Scholar
  15. Vandaele, S., Fosset, M., Galizzi, J. P., and Lazdunski, M., 1987, Monoclonal antibodies that coimmunoprecipitate the 1,4-dihydropyridine and phenyalkylamine receptors and reveal the Ca2+ chan nel structure, Biochemistry 26:5–9.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Michel Fosset
    • 1
  • Sylvie Vandaele
    • 1
  • Jean-Pierre Galizzi
    • 1
  • Jacques Barhanin
    • 1
  • Michel Lazdunski
    • 1
  1. 1.Center for BiochemistryCNRSNice CedexFrance

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