Advertisement

Phospholipid-Calcium Relations at the Sarcolemma of the Cardiac Cell

Their Possible Role in Control of Contractility
  • G. A. Langer
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

The mammalian heart controls its force development by two general mechanisms: (1) The Frank-Starling response to changing preload which involves a change in diastolic fiber length, and (2) a change of contractile state in which force changes without a primary change in the relation of sarcomeric myofilaments to each other. There is evidence (Allen and Kentish, 1985) that the Frank-Starling response might involve changes in intracellular calcium (Ca) distribution and/or myofilament sensitivity that may affect force, but the primary event is the length change. In contrast, the second mechanism, change in contractile state, is primarily dependent on alterations in cellular Ca flux and exchange. The characteristics of this flux and exchange have been the focus of my laboratory at UCLA for the past 20 years. This paper will present a brief review of the current state of our knowledge with emphasis on the possible role of phospholipid in the sarcolemmal membrane in the regulation of Ca binding and exchange.

Keywords

Contractile Force Contractile State Diffuse Double Layer Sarcolemmal Membrane Exchangeable Pool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allen, D. G., and Kentish, J. C., 1985, The cellular basis of the length-tension relation in cardiac muscle, J. Mol. Cell. Cardiol. 17:821–840.PubMedCrossRefGoogle Scholar
  2. Barry, W. H., and Smith, T. W., 1982, Mechanisms of transmembrane calcium movement in cultured chick embryo ventricular cells, J. Physiol. 325:243–260.PubMedGoogle Scholar
  3. Bers, D. M., and Langer, G. A., 1979, Uncoupling cation effects on cardiac contractility and sarco-lemmal Ca2+ binding, Am. J. Physiol. 237:H332–H341.PubMedGoogle Scholar
  4. Burt, J. M., and Langer, G. A., 1983, Ca2+ displacement of polymyxin B from sarcolemma isolated by “gas dissection” from cultured neonatal rat myocardial cells, Biochem. Biophys. Acta. 729: 44–52.PubMedCrossRefGoogle Scholar
  5. Burt, J. M., Duenas, C. J., and Langer, G. A., 1983, Influence of polymyxin B, a probe for anionic phospholipids, on calcium binding and calcium and potassium fluxes of cultured cardiac cells, Circ. Res. 53:679–687.PubMedGoogle Scholar
  6. Burt, J. M., Rich, T. L., and Langer, G. A., 1984, Phospholipase D increases cell surface Ca2+ binding and positive inotropy in rat heart, Am. J. Physiol. 247:H880–H885.PubMedGoogle Scholar
  7. Caroni, P., Zurini, M., Clark, A., and Carafoli, E., 1983, Further characterization and reconstitution of the purified Ca2+ -pumping ATPase of heart sarcolemma, J. Biol. Chem. 258:7305–7310.PubMedGoogle Scholar
  8. Fintel, M., Langer, G. A., Rohloff, J. C., and Jung, M., 1985, Contribution of myocardial diffuse double layer calcium to contractile function, Am. J. Physiol. 249:H989–H994.PubMedGoogle Scholar
  9. Langer, G. A., and Frank, J. S., 1972, Lanthanum in heart cell culture, J. Cell. Biol. 54:441–455.PubMedCrossRefGoogle Scholar
  10. Langer, G. A., 1985, The effect of pH on cellular and membrane calcium binding and contraction of myocardium. A possible role for sarcolemmal phospholipid in EC coupling, Circ. Res. 57:374–382.PubMedGoogle Scholar
  11. Langer, G. A., and Nudd, L. M., 1983, Effects of cations, phospholipases and neuraminidase on calcium binding to “gas dissected” membranes from cultured cardiac cells, Circ. Res. 53:482–490.PubMedGoogle Scholar
  12. Langer, G. A., and Rich, T. L., 1985, Phospholipase D produces increased contractile force in rabbit ventricular muscle, Circ. Res. 56:146–149.PubMedGoogle Scholar
  13. Langer, G. A., and Rich, T. L., 1986, Augmentation of sarcolemmal Ca by anionic amphiphile: Contractile response of three ventricular tissues, Am. J. Physiol. 250:H247–H254.PubMedGoogle Scholar
  14. Langer, G. A., Frank, J. S., and Nudd, L. M., 1979, Correlation of calcium exchange, structure, and function in myocardial tissue culture, Am. J. Physiol. 237:H239–H246.PubMedGoogle Scholar
  15. Langer, G. A., Frank, J. S., Rich, T. L., and Orner, F. B., 1987, Calcium exchange, structure, and function in cultured adult myocardial cells, Am. J. Physiol. 252:H314–H324.PubMedGoogle Scholar
  16. Lee, K. S., Marban, E., and Tsien, R. W., 1985, Inactivation of calcium channels in mammalian heart cells: joint dependence on membrane potential and intracellular calcium, J. Physiol. 364:395–411.PubMedGoogle Scholar
  17. McLaughlin, A., Eng, W., Vaio, G., Wilson, T., and McLaughlin, S., 1983, Dimethonium, a divalent cation that exerts only a screening effect on the electrostatic potential adjacent to negatively charged phospholipid bilayer membranes, J. Membrane Biol. 76:183–193.CrossRefGoogle Scholar
  18. Philipson, K. D., and Nishimoto, A. Y., 1984, Stimulation of Na + -Ca2+ exchange in cardiac sarcolemmal vesicles by phospholipase D, J. Biol. Chem. 259:16–19.PubMedGoogle Scholar
  19. Philipson, K. D., Bers, D. M., and Nishimoto, A. Y., 1980, The role of phospholipids in the Ca2+ binding of isolated cardiac sarcolemma, J. Mol. Cell. Cardiol. 12:1159–1173.PubMedCrossRefGoogle Scholar
  20. Philipson, K. D., Langer, G. A., and Rich, T. L., 1985, Charged amphiphiles regulate heart contractility and sarcolemmal Ca2+ interactions, Am. J. Physiol. 249:H147–H150.Google Scholar
  21. Sixl, F., and Galla, H. J., 1981, Polymyxin interaction with negatively charged lipid bilayer membranes and the competitive effect of Ca2+ , Biochim. Biophys. Acta. 643:626–635.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • G. A. Langer
    • 1
  1. 1.Cardiovascular Research LaboratoriesUniversity of California, Los Angeles School of Medicine, Center for the Health SciencesLos AngelesUSA

Personalised recommendations