Insulin-like Growth Factor II Stimulates Calcium Influx in Competent Balb/c 3T3 Cells Primed with Epidermal Growth Factor

  • Ikuo Nishimoto
  • Etsuro Ogata
  • Itaru Kojima
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Insulin-like growth factors (IGFs) are potent mitogens in mammalian cells (Zapfet al., 1981). The mode of action of IGFs has been extensively studied in Balb/c 3T3 cells. Thus, IGFs promote cell cycle progression specifically in competent Balb/c 3T3 cells while IGFs have essentially no effect in Go-arrested cells (Pledgeret al., 1977; Stileset al., 1979). To promote cell cycle progression, IGFs should be exposed to competent cells continuously throughout the Giphase (Pledgeret al., 1977). Hence, it is presumed that IGFs may generate a continuous mitogenic signal. This observation together with the fact that extracellular calcium is required for cell cycle progression have led to the consideration that calcium influx may be a mitogenic signal of IGFs.


Competent Cell Calcium Influx Thymidine Incorporation Extracellular Calcium Quiescent Cell 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Ikuo Nishimoto
    • 1
  • Etsuro Ogata
    • 1
  • Itaru Kojima
    • 1
  1. 1.Cell Biology Research Unit, Fourth Department of Internal MedicineUniversity of Tokyo School of MedicineBunkyoku, Tokyo 112Japan

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