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Growth Factors, Oncogenes, and Protein Kinase C

  • Ian G. Macara
  • George Gray
  • James Gaut
  • Anna Coco
  • Theresa Wingrove
  • Donna Faletto
  • Alan Wolfman
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Subconfluent cultures of 3T3 fibroblasts were labeled to isotopic equilibrium with [3 H]glycerol and analyzed by thin-layer chromatography after extraction of lipids to determine the levels of [3 H]diacylglycerol (DAG) in comparison to the total amount of3 H incorporated into cellular lipid. The transformed cells used in this study were always compared to their true parental clone and were of recent origin to decrease the possibility that observed changes are merely reflecting long-term cell divergence.

Keywords

Phorbol Ester Inositol Phosphate Inositol Monophosphate Revertant Cell Phosphatidylinositol Turnover 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Ian G. Macara
    • 1
  • George Gray
    • 1
  • James Gaut
    • 1
  • Anna Coco
    • 1
  • Theresa Wingrove
    • 1
  • Donna Faletto
    • 1
  • Alan Wolfman
    • 1
  1. 1.Environmental Health Sciences Center, Department of BiophysicsUniversity of Rochester Medical CenterRochesterUSA

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