Measurement of Free Intracellular Calcium (Cai) in Fibroblasts
Measurement of intracellular calcium has long been a goal in many areas of biology. Calcium has been heralded as an intracellular messenger because of its unique structure and binding properties (Kretsingeret al., 1980), which allow Ca2+ ion to bind and activate specific regulatory molecules, such as calmodulin (Cheung, 1980). Ca2+ -activated processes have been implicated in signal transduction in metabolism, neural transmission, excitation-con traction coupling, and mitosis, and, more recently, in the stimulation of early mitogenic events in cells as diverse as fibroblasts (Boyntonet al., 1980; Chafouleaset al., 1982; Morriset al., 1984; McNeilet al., 1985; Tuckeret al., 1986) and lymphocytes (Heskethet al., l983;Gelfandet al., 1986).
KeywordsExcitation Spectrum Free Calcium Digital Image Analysis Cytoplasmic Free Calcium Thick Ascend Limb Cell
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