Protein Kinase C in Rat Skeletal Muscle
Activation of skeletal muscle contraction under physiological conditions involves depolari zation of transverse tubular regions of the sarcolemma (Huxley and Taylor, 1958; Hodgkin and Horowicz, 1960; Costantin and Taylor, 1973; Costantin, 1975) leading to Ca2+ release from the terminal cisternae of the sarcoplasmic reticulum (Stephenson, 1981). The transverse tubular-terminal cisternae transmembrane signaling mechanism apparently is nonelectrical (Donaldson, 1985; Donaldsonet al., 1987 and references therein) and there have been numerous studies aimed at examining the chemical triggering of sarcoplasmic reticulum Ca2+ release. In this regard some researchers have examined the role of inositol phosphates in the mobilization of intracellular skeletal muscle Ca2+ (Volpeet al., 1985; Vergaraet al., 1985; Thieleczek and Heilmeyer, 1986; Noseket al., 1986; Donaldsonet al., 1987). Such studies were based on findings that inositol trisphosphate (IP3) elicited Ca2+ release from the endoplasmic reticulum of a wide variety of cells (Berridge, 1981; Suematsuet al., 1984; Hirataet al., 1984; Hokin, 1985). A recent study showed that locally applied microinjected 1µM IP3stimulated Ca2+ release from peeled skeletal muscle fibers and, although it did not sdirectly activate the contractile apparatus (Donaldsonet al., 1987), its role in excitation contraction coupling was concluded to involve propagation of Ca2+ release acting beyond the step of transverse tubule depolarization (Donaldsonet al., 1987) at the sarcoplasmic reticulum (Volpeet al., 1985).
KeywordsSarcoplasmic Reticulum Soleus Muscle Particulate Fraction Particulate Activity Inositol Trisphosphate
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- Hodgkin, A. L., and Horowicz, P., 1960, Potassium contractures in single muscle fibres, J. Physiol. (Lond.) 153:386–403.Google Scholar
- Huxley, A F., and Taylor, R. E., 1958, Local activation of striated muscle fibres, J. Physiol. (Lond.) 144:426–441.Google Scholar
- Kuo, J. F., Andersson, R. G. G., Wise, B. C., Mackerlova, L., Salomonsson, L., Brackett, N. L., Katoh, N., Shoji, M., and Wrenn, R. W., 1980, Calcium-dependent protein kinase: Widespread occurrence in various tissues and phyla of the animal kingdom and comparison of effects of phospholipid, calmodulin, and trifluoperazine, Proc. Natl. Acad. Sci. USA 77:7039–7043.PubMedCrossRefGoogle Scholar