Reconstituted Influenza Virus Envelopes as a Potential Carrier for Fusion-Mediated Microinjection of Macromolecules into Living Cells

  • Abraham Loyter
  • Moshe Lapidot
Part of the NATO ASI Series book series (NSSA, volume 155)


The process of virus-membrane fusion mediates penetration of most enveloped virions into animal cells (White et al, 1983). Fusion between the viral envelopes and membrane of recipient cells results in microinjection of the viral nucleocapsid into the cells’ cytoplasm.


Influenza Virion Virus Envelope Trapping Efficiency Sendai Virus Intact Virion 
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  1. Bosch, F. X., Mayer, A., and Huang, R. T. C., 1980, Simple and rapid separation of orto-and paramyxovirus glycoprotein, Med.Microbiol. Immunol., 168:249.PubMedCrossRefGoogle Scholar
  2. Chejanovsky, N., and Loyter, A. 1985, Fusion between envelopes and biological membranes, J.Biol.Chem., 260:7911.PubMedGoogle Scholar
  3. Citovsky, V., Blumentahl, R., and Loyter, A., 1985, Fusion of Sendai virions with phosphatidylcholine-cholesterol liposomes reflects the viral activity required for fusion with biological membranes, FEBS Lett., 193:135.PubMedCrossRefGoogle Scholar
  4. Citovsky, V., Zakai, N., and Loyter, A., 1986, Specific requirement for liposome-associated sialoglycolipids, but not sialoglycoproteins, to allow lysis of phospholipid vesicles by Sandai virions, Exp.Cell Res. 166:279.PubMedCrossRefGoogle Scholar
  5. Gitman, A. G., Graessman, A., and Loyter, A., 1985, Targeting of loaded Sendai virus envelopes by covalenly attached insulin molecules to virus receptor-depleted cells: Fusion-mediated microinjection of Ricin A and Simian virus 40 DNA, Proc.Natl.Acad.Sci.,USA, 82:7309.PubMedCrossRefGoogle Scholar
  6. Hanks, J. H., and Wallace, J. H., 1958, Determination of cell viability, Proc.Soc.Exp.Biol.Med., 98:188.PubMedGoogle Scholar
  7. Haywood, A. M., and Boyer, B. P., 1984, Effect of lipid composition upon fusion of liposomes with Sendai virus membrane, Biochemistry 29:4161.CrossRefGoogle Scholar
  8. Hoekstra, D., de Boer, T., Klappe, K., and Wilschut, J., 1984, Fluorescence method for measuring the kinetics of fusion between biological membranes, Biochemistry 23:5675.PubMedCrossRefGoogle Scholar
  9. Huang, R. T. C., Wahn, K., Klenk, H. D. and Rott, R., 1980, The fusion of the Neuraminidase in membrane fusion induced by Myxoviruses, Virology 197:313.CrossRefGoogle Scholar
  10. Huang, R. T. C., Rott, R., and Klenk, H. D., 1981, Influenza viruses cause hemolysis and fusion of cells, Virology 110:243.PubMedCrossRefGoogle Scholar
  11. Kaneda, Y., Uchida, T., Kim, J., Ishiura, M., and Okada, Y., 1987, The improved efficient method for introducing macromelecules into cells using HVJ (Sendai virus) liposomes with gangliosides, Exp.Cell Res., 173:56.PubMedCrossRefGoogle Scholar
  12. Lapidot, M., Nussbaum, O., and Loyter, A., 1987, Fusion of membrane vesicles bearing only the influenza hemayglutinin with erythrocytes, living cultured cells, and liposomes, J.Biol.Chem. 262:13736.PubMedGoogle Scholar
  13. Loyter, A., and Volsky, D. J., 1982, Reconstituted Sendai virus envelopes as carriers for the introduction of biological material into animal cells, in: “Membrane Reconstitution,” Poste, G., and Nicolson, G. L., eds., Elsevier/North Holland, Biomedical Press, Amsterdam.Google Scholar
  14. Loyter, A., Gitman, A. G., Chejanovsky, N., and Nussbaum, O., 1986, Fusion-mediated microinjection of macromolecules into living cultured cells by “targeted” Sendai virus envelopes, in: “Microinjection and Organelle Transplantation Techniques,” J. E. Cellis, A. Graessman and. Loyter, eds., Academic Press, New York.Google Scholar
  15. Nussbaum, O., Lapidot, M., and Loyter, A., 1987, Reconstitution of functional influenza virus envelopes and fusion with membrane and liposome lacking virus receptors, J.Virol., 61:2245.PubMedGoogle Scholar
  16. Nussbaum, O., and Loyter, A., 1987, Quantitative determination of virus-membrane fusion events: Fusion of influenza virions with plasma membranes and membranes of endocyte besides in living cultured cells, FEBS Lett. 221:61.PubMedCrossRefGoogle Scholar
  17. Poste, G., and Pasternak, C. A., 1978, Virus induced cell fusion, in: “Cell Surface Reviews: Membrane Fusion,” G. Poste and G. L. Nicolson, eds., Vol. 5, North Holland Publishing Co., Amsterdam.Google Scholar
  18. Scherphof, G. L., Spanjer, H. H., Dijkstra, J., Derksen, J. T. P., and Roerdnik, F. H., 1986, Participation of Kupffer cells and Hepato-cytes, in: “Hepatic Uptake and Processing of Liposomes, in Medical Application of Liposomes,” K. Yagi, ed., Japan Scientific Societies Press, Tokyo.Google Scholar
  19. Stegmann, T., Hoekstra, O., Senerphob, G., and Wilschut, J., 1985, Kinetics of pH-dependent fusion between influenzy virus and liposomes, Biochemistry 24:3107.PubMedCrossRefGoogle Scholar
  20. Svensson, V., 1985, Role of vesicles during Adenovirus 2 internalization into Hela cells, J.Virol., 55:442.PubMedGoogle Scholar
  21. Szoka, F., and Papahadjoupoulos, D., 1978, Procedure for preparation of liposomes with large internal aqueous space an high capture by reverse phase evaporation, Proc.Natl.Acad.Sci.USA 75:4194.PubMedCrossRefGoogle Scholar
  22. Uchida, T., Kim, J., Yamaizumi, M., Miyake, Y., and Okada, Y., 1979, Reconstitution of lipid vesicles associated with HVJ (Sendai virus) spikes: Purification and some properties of vesicles containing non-toxic fragment A of diphtheria toxin, J.Cell Biol. 80:10.PubMedCrossRefGoogle Scholar
  23. Vainstein, A., Rzin, A., Graessmann, A., and Loyter, A., 1983, Fusogenic reconstituted Sendai virus envelopes as a vehicle for introduction DNA into viable mammalian cells, Meth. 101:492.Google Scholar
  24. Vainstein, A., Hershkovitz, M., Israel, S., Rabin, S., and Loyter, A., 1984, A new method for reconstitution of highly fusogenic Sendai virus envelopes, Biochim.Biophys.Acta 773:181.PubMedCrossRefGoogle Scholar
  25. White, J., Kielian, M., and Helenius, A., 1983, Membrane fusion proteins of enveloped animal viruses, Q.Rev.Biophys. 16:151.PubMedCrossRefGoogle Scholar
  26. Wiley, D. C., 1987, The structure and function of the Hemagglutinin membrane glycoprotein of Influenza virus, Ann.Rev.Biochem. 56:365.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Abraham Loyter
    • 1
  • Moshe Lapidot
    • 1
  1. 1.Department of Biological Chemistry, Institute of Life SciencesHebrew University of JerusalemJerusalemIsrael

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