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Phospholipid Stereospecificity in Liposomal Modulation of Nitrogen Mustard Action

  • Carl Ritter
  • Charles Prood
  • Robert J. Rutman
Part of the NATO ASI Series book series (NSSA, volume 155)

Abstract

Liposomes made of L(α) dipalmitoyl phosphatidyl choline (DPPC) enhance the antitumor efficacy of nitrogen mustard (HN2) in vivo, (Ritter et al, 1981) and in vitro (Ritter et al, 1987). Thus, liposomes act on the tumor cells directly rather than by altering NH2 distribution within the animal. In addition, since HN2 and DPPC liposomes do not associate, liposomes do not physically carry HN2 into tumor cells (Ritter et al, 1987). In lip-osome-treated cells though only slightly more HN2 is taken up, the amounts of cytolytic activity and DNA alkylation are doubled by comparison with HN2-treated controls (Ritter et al, 1987). Thus, liposomes increase the amount of HN2 distributed to the nucleus. By contrast, following Ca-H-treatment there is a 31% increase in cellular HN2 uptake but 50% decrease in DNA alkylation and cytolysis (Ritter et al, 1987). The effects on uptake seem to be much less important than the effects on intracellular distribution.

Keywords

Nitrogen Mustard DPPC Liposome Longterm Survivor Dipalmitoyl Phosphatidyl LIPOSOMAL Modulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Lee, E. T., 1980, “Statistical Methods for Survival Analysis,” Lifetime Learning Publications, Belmont.Google Scholar
  2. Miller, R. G., 1981, “Survival Analysis,” Wiley.Google Scholar
  3. Ritter, C., Iyengar, C. L., and Rutman, R. J., 1981, Differential enhancement of antitumor effectiveness by phospholipid vesicles (liposomes), Cancer Res., 41:2366.PubMedGoogle Scholar
  4. Ritter, C., Rutman, R. J., and Goldstein, N. O., 1987, Modulation of the cellular toxicity of nitrogen mustard in murine cells, Cancer Res. 47:472.PubMedGoogle Scholar
  5. Rutman, R. J., Steele, W. J., and Price, C. C., 1961, Experimental chemotherapy studies, I. Chemical and metabolic investigations of chloroquine mustard, Cancer Res., 21:1124.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Carl Ritter
    • 1
  • Charles Prood
    • 1
  • Robert J. Rutman
    • 1
  1. 1.Department of Animal Biology, School of Veterinary MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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