A Comparison of Methods for Analysis of mRNA in Hematopoietic Cells: Conventional and Clonal Northern Analysis and In Situ Hybridization

  • Cathy L. Castiglia
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 34)


Three methods for the detection of mRNA in hematopoietic cells are addressed, and examples using these techniques to examine human leukemic cells’ genetic response to chemical and biological response modifiers shown. The limitations and specific applications of the three techniques are discussed.


Acute Promyelocytic Leukemia Biological Response Modifier Cellular mRNA Human Promyelocytic Leukemia Cell Guanidinium Isothiocyanate 
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  1. 1.
    Paige, C.J., G. Wu, and C.L. Castiglia. 1986. Detection of RNA transcripts in normal lymphoid and myeloid colonies. J I mm Methods 93: 37.Google Scholar
  2. 2.
    Chirgwin, J.M., A.E. Przybyla, R.J. MacDonald, and W.J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochem 18: 5394.Google Scholar
  3. 3.
    Aviv, H., and P. Leder. 1972. Purification of biologically active globin messenger RNA by chromotography on oligothymidylic acid-cellulose. Proc Natl Acad Sci. 69: 1408.PubMedCrossRefGoogle Scholar
  4. 4.
    Carmichael, G.G., and G.K. McMaster. 1980. The analysis of nucleic acids in gels using glyoxal and acridine orange. Methods in Enz. 65: 380.CrossRefGoogle Scholar
  5. 5.
    Feinberg, A.P., and B. Vogelstein. 1984. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 137: 266.PubMedCrossRefGoogle Scholar
  6. 6.
    Holmes, D.S., and J. Bonner. 1973. Preparation, molecular weight, base composition, and secondary structure of giant nuclear ribonucleic acid. Biochem. 12: 2330.CrossRefGoogle Scholar
  7. 7.
    Gallagher, R., S. Collins, J. Trujillo, K. McCredie, M. Ahearn, S. Tsai, R. Metzgar, G. Aulakh, R. Tine, F. Ruscetti, and R. Gallo. 1979. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood 54: 713.PubMedGoogle Scholar
  8. 8.
    Platzer, E., K. Welte, J.L. Gabrilove, L. Lu, P. Harris, R. Mertelzmann, and M.A.S. Moore. 1985. Biological activities of a human pluripotent hemopoietic colony stimulating factor on normal and leukemic cells. J. Exp. Med. 162: 1788.PubMedCrossRefGoogle Scholar
  9. 9.
    Harris, P.E., P. Ralph, J.L. Gabrilove, K. Welte, R. Karmali, and M.A.S. Moore. 1985. Distinct differentiation-inducing activities of gamma-interferon and cytokine factors acting on the human promyelocytic leukemia cell line HL-60. Cancer Res. 45: 3090.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Cathy L. Castiglia
    • 1
  1. 1.Sloan-Kettering InstituteNew YorkUSA

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