Retroviral Gene Transfer: Applications to Human Therapy
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 34)
The understanding of gene expression has been greatly enhanced by the ability to transfer cloned genes into cells and to study the mechanism of their regulation. For the past several years it has been recognized that retroviruses are good candidates as vehicles, or vectors, to introduce genes into eucaryotic cells. Retrovirus-derived vectors utilize the biochemical processes unique to this group of viruses, to transfer genes with high efficiency into a wide variety of cell types, in vitro and in vivo. By using retrovirus derived vectors, the effect of newly introduced genes and the mechanism of gene expression can be studied in cell types so far refractory to gene transfer. The special features of this new gene transfer technology have provided for the first time the opportunity of introducing genes into the somatic cells of live animals. Although at present limited to gene transfer into hemopoietic cells, its potential in general studies and applications to human therapy is beginning to be recognized.
KeywordsInternal Promoter Human Therapy Umbilical Cord Blood Cell Gene Transfer Technology Human Umbilical Cord Blood Cell
Miller, D.A., E.S. Ong, M.G. Rosenfeld, I.M. Verma, and R.M. Evans. 1984. Infectious and Selectable retrovirus containing an inducible rat growth hormone minigene. Science
225: 993–997.PubMedCrossRefGoogle Scholar
Armentano, D., S.F. Yu, P.W. Kantoff, T. von Ruden, W.F. Anderson and E. Gilboa. 1987. Effect of Internal Viral Sequences on the Utility of Retroviral Vectors. J. Virol.
61: 1647–1650.PubMedGoogle Scholar
Zamecnik, P.C., and M.L. Stephenson. 1978. Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide. Proc. Natl. Acad. Sci.
75: 280–284.PubMedCrossRefGoogle Scholar
Izant, J.G., and H. Weintraub. 1984. Inhibition of thymidine kinase gene expression by anti-sense RNA: A molecular approach to genetic analysis. Cell
36: 1007–1015.PubMedCrossRefGoogle Scholar
Coleman, J., Green, P.J. and Inouye, M. 1984. The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes. Cell
37: 429–436.PubMedCrossRefGoogle Scholar
Pestka, S., B.L. Dougherty, V. Jung, K. Hoffa, and R.K. Pestka. 1984. Anti-mRNA: specific inhibition of translation of single mRNA molecules. Proc. Natl. Acad. Sci.
81: 7525–7528.PubMedCrossRefGoogle Scholar
Markham, P.D., S.Z. Salahuddin, V.S. Kalyanaraman, M. Popovic, P. Sarin, and R. Gallo. 1983. Infection and transformation of fresh human umbilical cord blood cells by multiple sources of Human T-Cell Leukemia Virus (HTLV). Int. J. Cancer.
31: 413–420.PubMedCrossRefGoogle Scholar
© Plenum Press, New York 1988