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Retroviral-Mediated Gene Transfer into Hemopoietic Cells

  • Martin A. Eglitis
  • Philip W. Kantoff
  • Donald B. Kohn
  • Evelyn Karson
  • Robert C. Moen
  • Clinton D. LothropJr.
  • R. Michael Blaese
  • W. French Anderson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 34)

Abstract

Retroviral vectors have provided a means for the introduction of functioning exogenous genes into the hematopoietic system of whole animals. Although these vectors are quite efficient in the mouse model, when applied to non-murine in vivo systems, the efficiency of gene transfer has diminished to impractical levels. Since in vivo analyses are expensive and time consuming, in vitro models have been developed to speed the evaluation of alternative protocols. Using in vitro colony assays, three approaches were evaluated for their ability to improve the infectivity of hematopoietic progenitor cells with retroviral vectors. Exogenously applied hematopoietic growth factors increased the proportion of hematopoietic colonies in vitro up to an average of 5 fold. When alternative sources of progenitors, such as fetal cord blood, were used, improvements in infection efficiency were also obtained. Finally, evidence was acquired suggesting that xenotropic packaging of vectors also improved infection efficiency.

Keywords

Hematopoietic Progenitor Hematopoietic Growth Factor Infection Efficiency Helper Virus Colony Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Martin A. Eglitis
    • 1
  • Philip W. Kantoff
    • 1
  • Donald B. Kohn
    • 2
  • Evelyn Karson
    • 1
  • Robert C. Moen
    • 1
  • Clinton D. LothropJr.
    • 3
  • R. Michael Blaese
    • 2
  • W. French Anderson
    • 1
  1. 1.Laboratory of Molecular Hematology, NHLBINIHBethesdaUSA
  2. 2.Metabolism Branch, NCINIHBethesdaUSA
  3. 3.Dept. of Environmental Practice, College of Veterinary MedicineUniversity of TennesseeKnoxvilleUSA

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