The Effects of Prostaglandin E1 on Megakaryocyte Proliferation In Vitro
A concentration of 560 nM Prostaglandin E1 (PGE1), in a medium containing either 10% serum and 10% plasma, or 20% plasma and a platelet extract prepared from 24 × 106 platelets/ml, caused after four days of incubation in a plasma clot culture system, highly significant increases over the controls in both the number of megakaryocyte colonies/clot and megakaryocytes/clot. A linear dose-response relationship over the entire 14–560 nM range was also demonstrated in cultures containing serum, between the concentration of PGE1 in each culture, and the number of megakaryocyte colonies/clot and megakaryocytes/clot. However, PGE1 did not appear to stimulate megakaryocytes in cultures containing only 20% rat plasma.
KeywordsIsotonic Saline Saline Control Plasma Clot Pokeweed Mitogen Platelet Extract
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