Tocopherol Behavior and Membrane Constituents in Erythrocytes with Oxidant Stress

  • Masayuki Miki
  • Tadashi Motoyama
  • Yoshito Takenaka
  • Makoto Mino
Part of the Basic Life Sciences book series (BLSC, volume 49)


Recently, toxicity caused by oxygen and its active species has received much attention in connection with a variety of pathological events.1 However, the mechanism responsible for the peroxidation of biomembranes is still unclear. Tocopherols (vitamin E) are located in the membranes and act there as an only chain-breaking antioxidant.2,3 When biomembranes are damaged mainly by a free radical chain oxidation,4 the effects of tocopherols will be spotlighted. Nevertheless, the mechanisms by which membrane tocopherols inhibit the peroxidation still require detailed study, partly because the active species and the sites of their generation have not usually been known or controlled.


Methyl Linoleate Peroxyl Radical Membrane Constituent Synergistic Inhibition Electron Spin Reso 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    W. Bors, M. Saran, and D. Tait, “Oxygen Radicals in Chemistry and Biology,” Walter de Gruyter, Berlin (1984).CrossRefGoogle Scholar
  2. 2.
    M. Mino, S. Nakagawa, H. Tamai, and M. Miki, Clinical evaluation of red blood cell tocopherol, Ann. NY Acad. Sci. 393:175 (1982).CrossRefGoogle Scholar
  3. 3.
    G.W. Burton, A. Joyce, and K.U. Ingold, Is vitamin E the only lipidsoluble, chain-breaking antioxidant in human blood plasma and erythrocyte membrane?, Arch. Biochem. Biophys. 221:281 (1983).PubMedCrossRefGoogle Scholar
  4. 4.
    M. Miki, H. Tamai, M. Mino, Y. Yamamoto, and E. Niki, Free-radical chain oxidation of rat red blood cells by molecular oxygen and its inhibition by α-tocopherol, Arch. Biochem. Biophys. 258:373 (1987).PubMedCrossRefGoogle Scholar
  5. 5.
    Y. Yamamoto, S. Haga, E. Niki, and Y. Kamiya, Oxidation of lipids. V. Oxidation of methyl linoleate in aqueous dispersion, Bull. Chem. Soc. Jpn. 57:1260 (1984).CrossRefGoogle Scholar
  6. 6.
    L.R.C. Barclay, S.J. Lccke, J.M. MacWeil, J. VanKessel, G.W. Burton, and K.U. Ingold, Autoxidation of micelles and model membranes. Quantitative kinetic measurements can be made by using either water-soluble or lipid-soluble initiators with water-soluble or lipid-soluble chain-breaking antioxidants, J. Am. Chem. Soc. 106:2479 (1984).CrossRefGoogle Scholar
  7. 7.
    G.W. Burton and K.U. Ingold, Autoxidation of biological molecules. 1. The antioxidant activity of vitamin E and related chain-breaking phenolic antioxidants in vitro, J. Am. Chem. Soc. 102:6472 (1981).CrossRefGoogle Scholar
  8. 8.
    E. Niki, J. Tsuchiya, Y. Yoshikawa, Y. Yamamoto, and Y. Kamiya, Oxidation of lipids. XIII. Antioxidant activities of α-, β-, γ-, and δ-tocopherols, Bull. Chem. Soc. Jpn. 59:497 (1986).CrossRefGoogle Scholar
  9. 9.
    E. Niki, Lipid antioxidants: How they may act in biological systems, Br. J. Cancer 55:153 (1987).CrossRefGoogle Scholar
  10. 10.
    E. Niki, T. Saito, A. Kawakami, and Y. Kamiya, Inhibition of oxidation of methyl linoleate in solution by vitamin E and vitamin C, J. Biol. Chem. 259:4177 (1984).PubMedGoogle Scholar
  11. 11.
    E. Niki, J. Tsuchiya, R. Tanimura, and Y. Kamiya, Regeneration of vitamin E from α-chromanoxyl radical by glutathione and vitamin C, Chem. Lett. 789 (1982).Google Scholar
  12. 12.
    E. Niki, A. Kawakami, Y. Yamamoto, and Y. Kamiya, Oxidation of lipids. VIII. Synergistic inhibition of oxidation of phosphatidylcholine liposome in aqueous dispersion by vitamin E and vitamin C, Bull. Chem. Soc. Jpn. 58:1971 (1985).CrossRefGoogle Scholar
  13. 13.
    T. Doba, G.W. Burton, and K.U. Ingold, Antioxidant and co-antioxidant activity of vitamin C. The effect of vitamin C, either alone or in the presence of vitamin E or a water-soluble vitamin E analogue, upon the peroxidation of aqueous multilamellar phospholipid liposomes, Biochim. Biophys. Acta 835:298 (1985).PubMedCrossRefGoogle Scholar
  14. 14.
    E. Niki, M. Saito, Y. Yoshikawa, Y. Yamamoto, and Y. Kamiya, Oxidation of lipids. XII. Inhibition of oxidation of soybean phosphatidylcholine and methyl linoleate in aqueous dispersions by uric acid, Bull. Chem. Soc. Jpn. 59:471 (1986).CrossRefGoogle Scholar
  15. 15.
    J. Tsuchiya, T. Yamada, E. Niki, and Y. Kamiya, Interaction of galvinoxyl radical with ascorbic acid, cysteine, and glutathione in homogeneous solution and in aqueous dispersions, Bull. Chem. Soc. Jpn. 58:326 (1985).CrossRefGoogle Scholar
  16. 16.
    E.A. Rachmilewitz, A. Shifter, and I. Kahane, Vitamin E deficiency in β-thalassemia major: Changes in hematological and biochemical parameters after a therapeutic trial with α-tocopherol, Am. J. Clin. Nutr. 32:1850 (1979).PubMedGoogle Scholar
  17. 17.
    P. Hochstein, and S.K. Jain, Association of lipid peroxidation and polymerization of membrane proteins with erythrocyte aging, Fed. Proc. 40:183 (1981).PubMedGoogle Scholar
  18. 18.
    J.F. Koster and R.G. Slee, Lipid peroxidation of human erythrocyte ghosts induced by organic hydroperoxides, Biochim. Biophys. Acta 752:233 (1983).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Masayuki Miki
    • 1
  • Tadashi Motoyama
    • 1
  • Yoshito Takenaka
    • 1
  • Makoto Mino
    • 1
  1. 1.Department of PediatricsOsaka Medical CollegeTakatsuki, Osaka 569Japan

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