Cytochrome P-448 Induction Restores the Propagation of •O2--Induced Lipid Peroxidation in Hepatoma Microsomes

  • Tommaso Galeotti
  • Silvia Borrello
Part of the Basic Life Sciences book series (BLSC, volume 49)


It is well established that intracellular and plasma membranes of cancer cells are resistant to lipid peroxidation and that such resistance is related directly to the growth rate and inversely to the degree of differentiation of the tumor.1 Lipid peroxidation byproducts are capable of inhibiting the mitotic activity of normal and tumor cells, 2,3 supporting the early hypothesis that the low peroxidizability of tumor membranes may be one of the factors responsible for the increased proliferative activity of malignant cells.4,5 Consistently, a reasonable approach to the study of lipid peroxidation in tumors is that of establishing the factors which limit the process and possibly finding strategies for restoring it at levels comparable to those of normal cells. The present and previous studies performed in our laboratory are aimed at carrying out this project.


Lipid Peroxidation Xanthine Oxidase Ehrlich Ascites Tumor Cell Ehrlich Ascites Tumor Italian National Research Council 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    T. Galeotti, S. Borrello, G. Minotti, and L. Masotti, Membrane alte rations in cancer cells: The role of oxy radicals, Ann. N.Y. Acad. Sci. 488:468 (1986)PubMedCrossRefGoogle Scholar
  2. 2.
    D. G. Cornwell and N. Marisaki, Fatty acids paradoxes in the control of cell proliferation: prostaglandins, lipid peroxides and cooxidation reactions, in: “Free Radicals in Biology,” Vol. 6, W. A. Pryor, ed., Academic Press, New York (1984).Google Scholar
  3. 3.
    S. Hauptlorenz, H. Esterbauer, W. Moll, R. Pumpel, E. Schauenstein, and B. Puschendorf, Effects of the lipid peroxidation product 4-hydroxy-nonenal and related aldehydes on proliferation and viability of cultured Ehrlich Ascites tumor cells, Biochem. Pharmacol. 34:3803 (1985).PubMedCrossRefGoogle Scholar
  4. 4.
    G. M. Bartoli and T. Galeotti, Growth-related lipid peroxidation in tumour microsomal membranes and mitochondria, Biochim. Biophys. Acta 574:537 (1979).PubMedCrossRefGoogle Scholar
  5. 5.
    T. Galeotti, G. M. Bartoli, S. Bartoli, and E. Bertoli, Superoxide radicals and lipid peroxidation in tumour microsomal membranes, in: “Biological and Clinical Aspects of Superoxide and Superoxide Dismutase,” W. H. Bannister and J. V. Bannister, eds., Elsevier, Amsterdam (1980).Google Scholar
  6. 6.
    H. P. Morris and B. P. Wagner, Induction and transplantation of rat hepatomas with different growth rate (including “minimal deviation” hepatomas), in.: “Methods in Cancer Research,” Vol. 4, H. Busch, ed., Academic Press, New York (1968).Google Scholar
  7. 7.
    S. Borrello, G. Minotti, G. Palombini, A. Grattagliano, and T. Galeotti, Superoxide-dependent lipid peroxidation and vitamin E content of microsomes from hepatomas with different growth rate, Arch. Biochem. Biophys. 238:588 (1985).PubMedCrossRefGoogle Scholar
  8. 8.
    T. Ohmachi, I. Sagami, H. Fujii, and M. Watanabe, Microsomal monooxygenase system in Morris hepatoma: Purification and characterization of cytochromes P-450 from Morris hepatoma 5124D of 3-methylcholanthrene-treated rats, Arch. Biochem. Biophys. 236:176 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    B. A. Svingen, J. A. Buege, F. O. O’Neal, and S. D. Aust, The mechanism of NADPH-dependent lipid peroxidation: The propagation of lipid peroxidation, J. Biol. Chem. 254:5892 (1979).PubMedGoogle Scholar
  10. 10.
    S. Borrello, T. Galeotti, G. Palombini, and G. Minotti, Restoration of hydroperoxide-dependent lipid peroxidation by 3-methylcholanthrene induction of cytochrome P-448 in hepatoma microsomes, FEBS Lett. 209:305 (1986).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Tommaso Galeotti
    • 1
  • Silvia Borrello
    • 1
  1. 1.Institute of General PathologyCatholic UniversityRomeItaly

Personalised recommendations