Determination of 8-Hydroxydeoxyguanosine in Human Urine: a Possible Assay for in Vivo Oxidative DNA Damage
A sensitive analytical method has been developed for the determination of 8-hydroxydeoxyguanosine (h8dG) in human urine, based on reversed-phase HPLC combined with electrochemical detection. Previous attempts to use urine as a means to monitor DNA oxidative damage have centered around the detection of thymine glycol, thymidine glycol, and 5-hydroxymethyluracil.1–3 The present assay offers improvements in detection limits, sample volumes and analysis time.
KeywordsHuman Urine Chronic Granulomatous Disease Sensitive Analytical Method Chronic Granulomatous Disease Patient Thymine Glycol
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- 2.R. L. Saul, P. Gee, and B. N. Ames, Free radicals, DNA damage, and aging, in: “Modern Biological Theories of Aging,” H. R. Warner et al., eds., Raven Press, New York (1987).Google Scholar
- 3.B. N. Ames and R. L. Saul, Cancer, aging, and oxidative DNA damage, in: “Theories of Carcinogenesis,” O. H. Iversen, ed., Hemisphere Publishing Corporation, Washington, D.C. (1988).Google Scholar
- 7.J. T. Curnette and B. M. Babior, in: “Advances in Human Genetics, Vol. 16,” H. Harris and K. Hirshhorn, eds., Plenum Publishing Corporation, New York (1987).Google Scholar