Advertisement

Determination of 8-Hydroxydeoxyguanosine in Human Urine: a Possible Assay for in Vivo Oxidative DNA Damage

  • Kenneth C. Cundy
  • Ron Kohen
  • Bruce N. Ames
Part of the Basic Life Sciences book series (BLSC, volume 49)

Abstract

A sensitive analytical method has been developed for the determination of 8-hydroxydeoxyguanosine (h8dG) in human urine, based on reversed-phase HPLC combined with electrochemical detection. Previous attempts to use urine as a means to monitor DNA oxidative damage have centered around the detection of thymine glycol, thymidine glycol, and 5-hydroxymethyluracil.1–3 The present assay offers improvements in detection limits, sample volumes and analysis time.

Keywords

Human Urine Chronic Granulomatous Disease Sensitive Analytical Method Chronic Granulomatous Disease Patient Thymine Glycol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    R. Cathcart, E. Schwiers, R. L. Saul, and B. N. Ames, Thymine glycol and thymidine glycol in human and rat urine: A possible assay for oxidative DNA damage, Proc. Natl. Acad. Sci. U.S.A. 81:5633 (1984).PubMedCrossRefGoogle Scholar
  2. 2.
    R. L. Saul, P. Gee, and B. N. Ames, Free radicals, DNA damage, and aging, in: “Modern Biological Theories of Aging,” H. R. Warner et al., eds., Raven Press, New York (1987).Google Scholar
  3. 3.
    B. N. Ames and R. L. Saul, Cancer, aging, and oxidative DNA damage, in: “Theories of Carcinogenesis,” O. H. Iversen, ed., Hemisphere Publishing Corporation, Washington, D.C. (1988).Google Scholar
  4. 4.
    H. Kasai, P. F. Crain, Y. Kuchino, S. Nishimura, A. Ootsuyama, and H. Tanooka, Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair, Carcinogenesis 7:1849 (1986).PubMedCrossRefGoogle Scholar
  5. 5.
    Y. Kuchino, F. Mori, H. Kasai, H. Inoue, S. Iwai, K. Miura, E. Ohtsuka, and S. Nishimura, Misreading of DNA templates containing 8-hydroxydeoxyguanosine at the modified base and at adjacent residues, Nature 327:77 (1987).PubMedCrossRefGoogle Scholar
  6. 6.
    R. A. Floyd, J. J. Watson, and P. K. Wong, Hydroxyl free radical adduct of deoxyguanosine: Sensitive detection and mechanisms of formation, Free Rad. Res. Commun. 1:163 (1986).CrossRefGoogle Scholar
  7. 7.
    J. T. Curnette and B. M. Babior, in: “Advances in Human Genetics, Vol. 16,” H. Harris and K. Hirshhorn, eds., Plenum Publishing Corporation, New York (1987).Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Kenneth C. Cundy
    • 1
  • Ron Kohen
    • 1
  • Bruce N. Ames
    • 1
  1. 1.Department of BiochemistryUniversity of CaliforniaBerkeleyUSA

Personalised recommendations