Determination of 8-Hydroxydeoxyguanosine in Human Urine: a Possible Assay for in Vivo Oxidative DNA Damage

  • Kenneth C. Cundy
  • Ron Kohen
  • Bruce N. Ames
Part of the Basic Life Sciences book series (BLSC, volume 49)


A sensitive analytical method has been developed for the determination of 8-hydroxydeoxyguanosine (h8dG) in human urine, based on reversed-phase HPLC combined with electrochemical detection. Previous attempts to use urine as a means to monitor DNA oxidative damage have centered around the detection of thymine glycol, thymidine glycol, and 5-hydroxymethyluracil.1–3 The present assay offers improvements in detection limits, sample volumes and analysis time.


Human Urine Chronic Granulomatous Disease Sensitive Analytical Method Chronic Granulomatous Disease Patient Thymine Glycol 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Kenneth C. Cundy
    • 1
  • Ron Kohen
    • 1
  • Bruce N. Ames
    • 1
  1. 1.Department of BiochemistryUniversity of CaliforniaBerkeleyUSA

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