Determination of 8-Hydroxydeoxyguanosine in Human Urine: a Possible Assay for in Vivo Oxidative DNA Damage
A sensitive analytical method has been developed for the determination of 8-hydroxydeoxyguanosine (h8dG) in human urine, based on reversed-phase HPLC combined with electrochemical detection. Previous attempts to use urine as a means to monitor DNA oxidative damage have centered around the detection of thymine glycol, thymidine glycol, and 5-hydroxymethyluracil.1–3 The present assay offers improvements in detection limits, sample volumes and analysis time.
KeywordsHPLC HYdroxyl Creatinine Glycol Adduct
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