Oxidative Damage to DNA in Adrenocortical Cells During Senescence in Culture

  • Kim H. Norris
  • Peter J. Hornsby
Part of the Basic Life Sciences book series (BLSC, volume 49)


Our experiments on the effects of oxidative damage on the functions of adrenocortical cells in culture were initiated by the finding that the loss of activity of cytochrome P-450 enzymes in cultured bovine adrenocortical cells incubated with steroid pseudosubstrates involved oxidative damage.1 Oxidative damage potentially occurs at a higher rate in cell culture than in vivo. Cultured cells are exposed to several potentially damaging adventitious oxidants under standard culture conditions, as well as oxidants from intracellular metabolic. processes.2 Moreover, we showed that cultured bovine adrenocortical cells suffer from a combined deficiency of vitamin E and selenium. The deficiency of these biological antioxidants in culture was observed as the ability of low concentrations of either nutrient to prevent the toxicity of the aminotransferase inhibitor, aminooxyacetate, and of an organic peroxide, cumene hydroperoxide.3,4 The two nutrients must be considered together since to a large extent the spectra of their biological activities overlap.5 Thus, it appeared worthwhile to investigate the possible role of oxidative damage in senescence of bovine adrenocortical cells in culture.


Oxidative Damage Down Syndrome Long Terminal Repeat Sister Chromatid Exchange Cloning Efficiency 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Kim H. Norris
    • 1
  • Peter J. Hornsby
    • 1
  1. 1.Department of Cell and Molecular BiologyMedical College of GeorgiaAugustaUSA

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