Sonochemistry of Nucleic Acid Constituents in Aqueous Solution: A Spin-Trapping Study

  • Takashi Kondo
  • C. Murali Krishna
  • Peter Riesz
Part of the Basic Life Sciences book series (BLSC, volume 49)


Ultrasonic cavitation in aqueous solution is well known to form free radical species produced by the collapse of cavitation bubbles. In this free radical formation, ultrasound is analogous to ionizing radiation.1,2 The study of the free radical effects of ultrasound on biological systems is of interest because of the widespread application of ultrasound in medical diagnosis and therapy. A recent ESR spin-trapping study has demonstrated that ·OH and H· are formed in water decomposition induced by cavitation.3 Some reports also have demonstrated stable sonoproducts arising from nucleic acid base destruction induced by ultrasound.4-6 However, direct evidence for the formation of nucleic acid base radicals was not obtained. In the present work, a water-soluble, non-volatile, aromatic nitroso spin trap, 3,5-dibromo-4-nitrosobenzenesulfonate (DBNBS),7 has been used for detecting the intermediate radicals arising from sonolysis of nucleic acid constituents, and the results have been compared with those obtained from H2O2-photolysis experiments in which only ·OH radicals are the reactive species.


Electron Spin Resonance Cavitation Bubble Spin Trap Sodium Formate Spin Adduct 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Takashi Kondo
    • 2
  • C. Murali Krishna
    • 1
  • Peter Riesz
    • 1
  1. 1.Radiation Oncology Branch, National Cancer InstituteNational Institutes of HealthBethesdaUSA
  2. 2.Department of Experimental Radiology and Health PhysicsFukui Medical SchoolMatsuoka, Fukui 910-11Japan

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