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Effect of ·OH Scavenging on the Yield of Radiation-Induced DNA Double-Strand Breaks in Isolated Nuclei

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Oxygen Radicals in Biology and Medicine

Part of the book series: Basic Life Sciences ((BLSC,volume 49))

Abstract

Isolated nuclei from Chinese hamster ovary cells were used to study the importance of ·OH scavenging in protection against the formation of locally multiply damaged sites (LMDS) by ionizing radiation. This system was selected because washed nuclei should be relatively free of low molecular weight endogenous OH scavengers. LMDS were assayed as DNA double-strand breaks (DSB) by neutral filter elution. The results are compared to the predictions of a model which considers ·OH radical diffusion, recombination, reaction with scavenger, and reaction with DNA.

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References

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Authors and Affiliations

  1. Joint Center for Radiation Therapy, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Edward A. Bump, Susan M. Pierce, Ceferino H. Obcemea, Lee Chin & C. Norman Coleman

Authors
  1. Edward A. Bump
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  2. Susan M. Pierce
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  3. Ceferino H. Obcemea
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  4. Lee Chin
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  5. C. Norman Coleman
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Editor information

Editors and Affiliations

  1. National Bureau of Standards, Gaithersburg, Maryland, USA

    Michael G. Simic

  2. International Life Sciences Institute, Washington, D.C., USA

    Karen A. Taylor

  3. Univesity of California, San Diego, La Jolla, California, USA

    John F. Ward

  4. Max-Planck-Institut für Strahlenchemie, Mülheim a.d. Ruhr, Federal Republic of Germany

    Clemens von Sonntag

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© 1988 Plenum Press, New York

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Bump, E.A., Pierce, S.M., Obcemea, C.H., Chin, L., Coleman, C.N. (1988). Effect of ·OH Scavenging on the Yield of Radiation-Induced DNA Double-Strand Breaks in Isolated Nuclei. In: Simic, M.G., Taylor, K.A., Ward, J.F., von Sonntag, C. (eds) Oxygen Radicals in Biology and Medicine. Basic Life Sciences, vol 49. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5568-7_65

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  • DOI: https://doi.org/10.1007/978-1-4684-5568-7_65

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5570-0

  • Online ISBN: 978-1-4684-5568-7

  • eBook Packages: Springer Book Archive

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