Effect of ·OH Scavenging on the Yield of Radiation-Induced DNA Double-Strand Breaks in Isolated Nuclei
Isolated nuclei from Chinese hamster ovary cells were used to study the importance of ·OH scavenging in protection against the formation of locally multiply damaged sites (LMDS) by ionizing radiation. This system was selected because washed nuclei should be relatively free of low molecular weight endogenous OH scavengers. LMDS were assayed as DNA double-strand breaks (DSB) by neutral filter elution. The results are compared to the predictions of a model which considers ·OH radical diffusion, recombination, reaction with scavenger, and reaction with DNA.
KeywordsProtection Factor Chinese Hamster Ovary Cell Radical Diffusion Isolate Nucleus Packed Region
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