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Effect of ·OH Scavenging on the Yield of Radiation-Induced DNA Double-Strand Breaks in Isolated Nuclei

  • Edward A. Bump
  • Susan M. Pierce
  • Ceferino H. Obcemea
  • Lee Chin
  • C. Norman Coleman
Part of the Basic Life Sciences book series (BLSC, volume 49)

Abstract

Isolated nuclei from Chinese hamster ovary cells were used to study the importance of ·OH scavenging in protection against the formation of locally multiply damaged sites (LMDS) by ionizing radiation. This system was selected because washed nuclei should be relatively free of low molecular weight endogenous OH scavengers. LMDS were assayed as DNA double-strand breaks (DSB) by neutral filter elution. The results are compared to the predictions of a model which considers ·OH radical diffusion, recombination, reaction with scavenger, and reaction with DNA.

Keywords

Protection Factor Chinese Hamster Ovary Cell Radical Diffusion Isolate Nucleus Packed Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Edward A. Bump
    • 1
  • Susan M. Pierce
    • 1
  • Ceferino H. Obcemea
    • 1
  • Lee Chin
    • 1
  • C. Norman Coleman
    • 1
  1. 1.Joint Center for Radiation TherapyHarvard Medical SchoolBostonUSA

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