Isolation and Partial Characterization of an Oxidized LDL in Humans

  • Pietro Avogaro
  • Gabriele Bittolo Bon
  • Giuseppe Cazzolato
Part of the Basic Life Sciences book series (BLSC, volume 49)


Foam cells that accumulate in the earliest of atherosclerotic lesions, the so-called fatty streak, arise from two cellular sources: the arterial smooth muscle cells (SMC) and the monocyte-derived macrophage (MM).2 The latter cell type in culture takes up only a small amount of native low density lipoproteins (LDL) by receptor-mediated endocytosis, but has a distinct receptor system that binds and degrades the more negatively charged LDL.2,3 Incubation of cultured MM with LDL modified by acetylation (acyl-LDL) results in an accumulation of cholesteryl esters (CE) within the cells thus forming foam cells.2 However such chemical modification in vivo seems unlikely. Recently, two possible mechanisms by which the more negatively charged LDL can be produced in vivo have been reported. The interaction of LDL with malondialdehyde (MDA) released by aggregating platelets or produced by peroxidation of fatty acids can lead to the formation of MDA-LDL that increase the CE deposition in cultured MM.4 Moreover, the interaction of LDL with endothelial cells also alters LDL (EC-LDL), thus allowing their uptake by the MM.5


Cholesteryl Ester Arterial Smooth Muscle Cell Cholesteryl Ester Concentration Conjugate Diene Product Isocratic High Performance Liquid Chromatography 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Pietro Avogaro
    • 1
  • Gabriele Bittolo Bon
    • 1
  • Giuseppe Cazzolato
    • 1
  1. 1.Unit for AtherosclerosisGeneral Regional HospitalVeniceItaly

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