Oxy Radical-Initiated Protein S-Thiolation and Enzymic Dethiolation

  • James A. Thomas
  • Eun-Mi Park
Part of the Basic Life Sciences book series (BLSC, volume 49)


It has often been suggested that protein S-thiolation/dethiolation (Figure 1) is an important form of metabolic regulation 1,2 . This suggestion usually arises from experiments in which a thiol-disulfide exchange mechanism is considered to be the basis for this process. Since cellular glutathione is normally very reduced, the thiol-disulfide exchange mechanism requires a significant oxidation of this thiol to account for appreciable protein modification in vivo. In addition, both S-thiolation and dethiolation by thiol-disulfide exchange probably require catalysis to occur at significant rates in vivo. These considerations make thiol-disulfide exchange less appealing than a direct oxy radical-initiated mechanism of S-thiolation. This paper describes experiments with electrofocusing technology to assess various mechanisms of protein S-thiolation and dethiolation.


Creatine Kinase Xanthine Oxidase Reduce Glutathione Glutathione Oxidation Glycogen Phosphorylase 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • James A. Thomas
    • 1
  • Eun-Mi Park
    • 1
  1. 1.Department of Biochemistry and BiophysicsIowa State UniversityAmesUSA

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