Implication of Protein Oxidation in Protein Turnover, Aging, and Oxygen Toxicity
A number of enzymatic and nonenzymatic mixed-function oxidation (MFO) systems catalyze the oxidation of enzymes.1–6 With oxidation, the enzymes are converted to catalytically inactive or less active forms that are more sensitive to heat denaturat ion7 and to proteolytic degradation.8–11 Enzyme oxidation is likely the basis of some normal biological functions, e.g., in the killing of bacteria by neutrophils, in limitation of the inflammatory response, in controlling the switch from anaerobic to aerobic metabolism, and in the regulation of the turnover of some enzymes. Enzyme oxidation also might be implicated in a variety of pathological disorders, including arthritis, aging, carcinogenesis, broncho-pulmonary dysplasia and adult respiratory distress syndrome, retinopathy of prematurity, and reperfusion-mediated ischemic damage.
KeywordsGlutamine Synthetase Protein Oxidation Histidine Residue Adult Respiratory Distress Syndrome Metal Binding Site
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- 2.C. N. Oliver, R. L. Levine, and E. R. Stadtman, Regulation of glutamine synthetase degradation, in: “Experiences in Biochemical Perception,” L.N. Ornston and S. G. Sligar, eds., Academic Press, New York (1982).Google Scholar
- 8.C. N. Oliver, R. Fulks, R. L. Levine, L. Fucci, A. J. Rivett, J. E. Roseman, and E. R. Stadtman, Oxidative inactivation of key metabolic enzymes during aging, in: “Molecular Basis of Aging,” A. K. Roy and B. Chatterjee, eds., Academic Press, New York (1984).Google Scholar
- 10.A. J. Rivett, J. E. Roseman, C. N. Oliver, R. L. Levine, and E. R. Stadtman, Covalent modification of proteins by mixed-function oxidation: Recognition by intracellular proteases, in: “Intracellular Protein Catabolism,” E. A. Khairallah, J. S. Bond, and J. W. C. Bird, eds., Alan R. Liss, New York (1985).Google Scholar
- 12.C. N. Oliver, B. Ahn, M. E. Wittenberger, R. L. Levine, and E. R. Stadtman, Age-related alterations of enzymes may involve mixed-function oxidation reactions, in “Modification of Proteins during Aging,” R. C. Adelman and E. R. Dekker, eds., Alan R. Liss, New York (1985).Google Scholar
- 15.A. Amici, L. Tsai, and E. R. Stadtman, unpublished data.Google Scholar
- 17.M. Rothstein, Changes in enzymatic proteins during aging, in “Molecular Basis of Aging,” A. K. Roy and B. Chatterjee, eds., Academic Press, New York (1984).Google Scholar
- 18.D. L. Garland, personal communication.Google Scholar
- 19.P. E. Starke-Reed, C. N. Oliver, and E. R. Stadtman, Modification of hepatic proteins in rats exposed to high oxygen concentration, FASEB J. 1:36 (1987).Google Scholar
- 20.S. Goldstein and E. J. Moerman, Unstable enzymes in erythrocytes of a family with Hutchinson Gilfar progeria syndrome, In: “The Red Cell,” G. J. Brewer, eds., Alan R. Liss, New York (1978).Google Scholar