Gel State Chemiluminescence: An Artificial Electron Transport System
Crosslinked glucose oxidase (GO) and horseradish peroxidase (HRP) can mimic macrophage oxidative function in vivo in respect to tumor cells as targets.1–3 Both the flavoprotein of NADPH oxidase of macrophages and GO contain flavin adenine dinucleotide as their prosthetic group. HRP has a noncovalently bound hematoporphyrin IX prosthetic group as does the cytochrome b559 (green hemoprotein) of NADPH oxidase. Therefore, we anticipated a similarity in biochemical mechanism of action and chemi-luminescence (CL) between the semi-synthetic crosslinked GO and HRP and the macrophage NADPH oxidase.
KeywordsHuman Serum Albumin NADPH Oxidase Glucose Oxidase Prosthetic Group Flavin Adenine Dinucleotide
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- 1.J.L. Kiel, “Insoluble Crosslinked Cytotoxic Oxidase-Peroxidase System,” U.S. Patent 4,486,408. Dec 4, 1984 (filed Apr 7, 1981).Google Scholar