Chemiluminescence from Vitamin E-Deficient Erythrocyte Membranes Induced by Xanthine Oxidase Reaction

  • Hiroshi Yasuda
  • Masayuki Miki
  • Yoshito Takenaka
  • Hiroshi Tamai
  • Makoto Mino
Part of the Basic Life Sciences book series (BLSC, volume 49)


Ultraweak chemiluminescence assay is known to be highly sensitive for oxidative reactions involved in the process of lipid peroxidation.1–3 The determination of spontaneous chemiluminescence would become a useful noninvasive mean for studying the oxidative damage in biological systems.4,5 However, the spontaneous chemiluminescence from living tissues may be too weak to be detected and identified, since biological systems are complex structured and contain a lot of substances serving as radical scavengers. Furthermore, the precise mechanism by which chemiluminescence is released still requires detailed study. In the light of the above point, the oxidation of red blood cell (RBC) membranes serves as a good model to study the peroxidative damage in the biological membranes.6,7 With the hypoxanthine-xanthine oxidase (HX-XOD) reaction, RBC membranes were found to be oxidized and result in hemolysis,8,9 where vitamin E in the membranes acted as a potent chain-breaking antioxidant and inhibited the peroxidation of membrane lipids as well as hemolysis.10,11 We have additionally reported that chemiluminescence was observed in the reaction of HX-XOD with vitamin E-deficient RBC.12


Chemiluminescence Intensity Ghost Membrane TBARS Formation Dialuric Acid Spontaneous Chemiluminescence 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Hiroshi Yasuda
    • 1
  • Masayuki Miki
    • 1
  • Yoshito Takenaka
    • 1
  • Hiroshi Tamai
    • 1
  • Makoto Mino
    • 1
  1. 1.Department of Pediatrics Osaka Medical CollegeOsaka 569Japan

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