Abstract
Oxygen-centered free radicals have been detected directly with ESR in a variety of biological processes such as lipid autoxidation,1 the enzymatic formation of superoxide by xanthine oxidase,2 and hydroxyl radical formation by the Y-irradiation of ice.3 Since the common isotope of oxygen is spin-less, no nuclear hyperfine interaction is possible, and the g-value and any hydrogen hyperfine coupling provide the only criteria for distinguishing an oxygen-centered free radical from other free radicals. The greatest limitation on the direct detection of oxygen-centered free radicals, aside from their high reactivity, is their paramagnetic properties.
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Mason, R.P., Morehouse, K.M. (1988). Electron Spin Resonance Investigations of Oxygen-Centered Free Radicals in Biological Systems. In: Simic, M.G., Taylor, K.A., Ward, J.F., von Sonntag, C. (eds) Oxygen Radicals in Biology and Medicine. Basic Life Sciences, vol 49. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5568-7_3
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DOI: https://doi.org/10.1007/978-1-4684-5568-7_3
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