Method for Tracing Oxygen-18 in Vivo: Application to Ozone Dosimetry in Animals

  • Mourad Aissa
  • Gary E. Hatch
Part of the Basic Life Sciences book series (BLSC, volume 49)

Abstract

The lack of a long-lived radioactive isotope of oxygen has impeded tracing studies involving oxygen labels. The Environmental Protection Agency (EPA) has investigated new methods for tracing oxygen-18 in vivo 1,2 to detect reaction products in inhaled ozone (O3) and other pollutants in tissues and for investigating mechanisms of toxicity. This report describes a technique for exposing experimental animals to oxygen-18 labeled ozone (18Oз), then converting oxygen present in respiratory tissues to CO2 prior to isotope ratio mass spectrometry to determine the enrichment of oxygen-18 (18O) in the tissue. Since O3 toxicity is believed to arise from either direct reaction of O3 with tissue constituents or from initiation of free radical-induced autoxidations, the 18O-tracing techniques should be applicable to detecting these as well as other types of oxygen reactions. It was hoped that this approach would be more generally applicable than approaches in which a specific reaction product was extracted then analyzed for the presence of the label.

Keywords

Surfactant Toxicity Quartz Dioxide Ozone 

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References

  1. 1.
    J. M. Hayes and J. Santrock, Isotopic study of the inhalation toxicology of oxidants. National Technical Information Service No. PB86–109485. EPA #600/1–85/013 (1985).Google Scholar
  2. 2.
    J. Santrock and J.M. Hayes, Adaptation of the Unterzaucher procedure for determination comof oxygen-18 in organic substances. Anal. Chem. 59:119 (1987).CrossRefGoogle Scholar
  3. 3.
    E. Pella and B. Colmbo, Improved instrumental determination of oxygen in organic pounds. Anal. Chem. 44:1563 (1972).CrossRefGoogle Scholar
  4. 4.
    J. Santrock, S.A. Studley, and J.M. Hayes, Isotopic analyses based on the mass spectrum of carbon dioxide. Anal. Chem. 57:1444 (1985).PubMedCrossRefGoogle Scholar
  5. 5.
    I. Roberts and H.C. Urey, Kinetics of the exchange between benzoic acid and water. J. Am. Chem. Soc. 61:2580 (1939).CrossRefGoogle Scholar
  6. 6.
    J. Santrock and J.M. Hayes, Pyrolytic decarboxylation of aromatic acids as a means of isotopic analysis. Anal. Chem. 57:1441 (1985).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Mourad Aissa
    • 1
  • Gary E. Hatch
    • 2
  1. 1.Northrop Services, Inc. - Environmental SciencesResearch Triangle ParkUSA
  2. 2.Inhalation Toxicology Division of U.S. Environmental Protection AgencyResearch Triangle ParkUSA

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