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Chemiluminescence — High Performance Liquid Chromatography of Phosphatidylcholine Hydroperoxide in Human Blood Plasma

  • Teruo Miyazawa
  • Keiichi Yasuda
  • Kenshiro Fujimoto
  • Takashi Kaneda
Part of the Basic Life Sciences book series (BLSC, volume 49)

Abstract

Normal phase HPLC (high performance liquid chromatography) with a UV detection has been used frequently for analyzing phospholipid classes. (1, 2) However, hydroperoxide and its hydroxy derivative usually give almost the same retention time as that for corresponding unoxidized species. (3) We have shown previously that chemiluminescence (CL) detection is very advantageous in its specificity and sensitivity for hydroperoxides. (4, 5) To analyze the phosphatidylcholine hydroperoxide (PCOOH), we newly devised the chemiluminescence — HPLC (CL-HPLC) system, in which luminol-cytochrome с solution is used as a hydroperoxide-specific CL reagent. We applied this new method to confirm the presence of PCOOH in blood plasma of healthy donors.

Keywords

Lipid Hydroperoxide Hydroxy Derivative Normal Phase HPLC High Performance Liquid Chromatographic Separation Plasma Total Lipid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    W. S. M. Geurts Van Kessel, W. M. A. Hax, R. A. Damel and J. De Gier, High performance liquid chromatographic separation and direct ultraviolet detection of phospholipids, Biochim. Biophys. Acta 486:524 (1977).PubMedCrossRefGoogle Scholar
  2. 2.
    W. M. A. Hax and W. S. M. Geurts Van Kessel, High-performance liquid chromatographic separation and photometric detection of phospholipids, J. Chromatogr. 142:735 (1977).PubMedCrossRefGoogle Scholar
  3. 3.
    J. Terao, I. Asano and S. Matsushita, Preparation of hydroperoxy and hydroxy derivatives of rat liver phosphatidylcholine and phosphatidylethanolamine, Lipids 20:312 (1985).PubMedCrossRefGoogle Scholar
  4. 4.
    T. Miyazawa, K. Yasuda and K. Fujimoto, Chemiluminescence — high performance liquid chromatography of phosphatidylcholine hydroperoxide, Anal. Lett. 20:915 (1987).CrossRefGoogle Scholar
  5. 5.
    T. Miyazawa, K. Fujimoto and T. Kaneda, Detection of picomole levels in lipid hydroperoxides by a chemiluminescence assay, Agric. Biol. Chem. 51:2569 (1987).Google Scholar
  6. 6.
    H. Inaba, A. Yamagishi, C. Takyu, B. Yoda, Y. Goto, T. Miyazawa, T. Kaneda and A. Saeki, Development of an ultra-high sensitive photon counting system and its application to biomedical measurements, Optics and Lasers in Engineering 3:125 (1982).CrossRefGoogle Scholar
  7. 7.
    T. Kaneda and T. Miyazawa, Lipid peroxides and nutrition, Wld. Rev.Nutr. Diet. 50:186 (1987).Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Teruo Miyazawa
    • 1
  • Keiichi Yasuda
    • 1
  • Kenshiro Fujimoto
    • 1
  • Takashi Kaneda
    • 2
  1. 1.Department of Food ChemistryFaculty of Agriculture Tohoku UniversitySendai 980Japan
  2. 2.Kohriyama Women’s CollegeKohriyama 963Japan

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