Chemiluminescence — High Performance Liquid Chromatography of Phosphatidylcholine Hydroperoxide in Human Blood Plasma
Normal phase HPLC (high performance liquid chromatography) with a UV detection has been used frequently for analyzing phospholipid classes. (1, 2) However, hydroperoxide and its hydroxy derivative usually give almost the same retention time as that for corresponding unoxidized species. (3) We have shown previously that chemiluminescence (CL) detection is very advantageous in its specificity and sensitivity for hydroperoxides. (4, 5) To analyze the phosphatidylcholine hydroperoxide (PCOOH), we newly devised the chemiluminescence — HPLC (CL-HPLC) system, in which luminol-cytochrome с solution is used as a hydroperoxide-specific CL reagent. We applied this new method to confirm the presence of PCOOH in blood plasma of healthy donors.
KeywordsLipid Hydroperoxide Hydroxy Derivative Normal Phase HPLC High Performance Liquid Chromatographic Separation Plasma Total Lipid
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