Chemiluminescence — High Performance Liquid Chromatography of Phosphatidylcholine Hydroperoxide in Human Blood Plasma

  • Teruo Miyazawa
  • Keiichi Yasuda
  • Kenshiro Fujimoto
  • Takashi Kaneda
Part of the Basic Life Sciences book series (BLSC, volume 49)


Normal phase HPLC (high performance liquid chromatography) with a UV detection has been used frequently for analyzing phospholipid classes. (1, 2) However, hydroperoxide and its hydroxy derivative usually give almost the same retention time as that for corresponding unoxidized species. (3) We have shown previously that chemiluminescence (CL) detection is very advantageous in its specificity and sensitivity for hydroperoxides. (4, 5) To analyze the phosphatidylcholine hydroperoxide (PCOOH), we newly devised the chemiluminescence — HPLC (CL-HPLC) system, in which luminol-cytochrome с solution is used as a hydroperoxide-specific CL reagent. We applied this new method to confirm the presence of PCOOH in blood plasma of healthy donors.


Lipid Hydroperoxide Hydroxy Derivative Normal Phase HPLC High Performance Liquid Chromatographic Separation Plasma Total Lipid 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Teruo Miyazawa
    • 1
  • Keiichi Yasuda
    • 1
  • Kenshiro Fujimoto
    • 1
  • Takashi Kaneda
    • 2
  1. 1.Department of Food ChemistryFaculty of Agriculture Tohoku UniversitySendai 980Japan
  2. 2.Kohriyama Women’s CollegeKohriyama 963Japan

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