Direct Chemical Ionization Mass Spectrometry of Underivatized Arachidonic Acid Metabolites: Application to Leukotriene Biosynthesis
Leukotrienes are a group of endogenous compounds active in hypersensitivity and inflammations. Few reports describe the generation and metabolism of leukotrienes (in particular LTB4) by other than specialized cells (i.e., macrophages, neutrophils, basophils). However, hepatocytes, too, seem capable of biosynthesis and inactivation of leukotrienes. Our work on the possible involvement of HPETEs or leukotrienes in hepatotoxic processes required a mass spectral analysis method (MS) that allowed the structural elucidation of minimal amounts of these compounds after separation on HPLC.
KeywordsHuman Liver Microsome Fast Atom Bombardment Mass Spectral Silyl Ether Chemical Ionization Reagent
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- 1.R. Murphy, Eicosanoid mass spectrometry, Spectra 10:11 (1985).Google Scholar