Direct Chemical Ionization Mass Spectrometry of Underivatized Arachidonic Acid Metabolites: Application to Leukotriene Biosynthesis

  • Josef Gut
  • Gene C. Jamieson
  • James R. Trudell
Part of the Basic Life Sciences book series (BLSC, volume 49)


Leukotrienes are a group of endogenous compounds active in hypersensitivity and inflammations. Few reports describe the generation and metabolism of leukotrienes (in particular LTB4) by other than specialized cells (i.e., macrophages, neutrophils, basophils). However, hepatocytes, too, seem capable of biosynthesis and inactivation of leukotrienes. Our work on the possible involvement of HPETEs or leukotrienes in hepatotoxic processes required a mass spectral analysis method (MS) that allowed the structural elucidation of minimal amounts of these compounds after separation on HPLC.


Human Liver Microsome Fast Atom Bombardment Mass Spectral Silyl Ether Chemical Ionization Reagent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R. Murphy, Eicosanoid mass spectrometry, Spectra 10:11 (1985).Google Scholar
  2. 2.
    F. J. G. M. van Kuijk, E. W. Thomas, R. J. Stevens, and E. A. Dratz, Gas chromatography-mass spectrometry method for determination of phospholipid peroxides, L Free Rad. Biol. Med. 1:387 (1986).CrossRefGoogle Scholar
  3. 3.
    G. C. Jamieson, C. C. Reuter, and W. L. Fitch, Polyimide-coated fused silica as an extended probe for in-beam chemical ionization mass spectrometry, Anal. Chem. 57:121 (1985).CrossRefGoogle Scholar
  4. 4.
    J. Gut, D. W. Goldman, G. C. Jamieson, and J. R. Trudell, Conversion of leukotriene A4 to leukotriene B4: Catalysis by human liver microsomes under anaerobic conditions, Arch. Biochem. Biophys. 259:497 (1987).PubMedCrossRefGoogle Scholar
  5. 5.
    J. Gut, G. C. Jamieson, and J. R. Trudell, Quantification of 5- and 15-HPETEs by direct chemical ionization mass spectrometry, Free Rad. Biol. Med. 3:323 (1987).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Josef Gut
    • 1
  • Gene C. Jamieson
    • 2
  • James R. Trudell
    • 1
  1. 1.Department of AnesthesiaStanford University School of MedicineStanfordUSA
  2. 2.SunnyvaleUSA

Personalised recommendations