The Generation of Ferryl or Hydroxyl Radicals During Interaction of Hemeproteins with Hydrogen Peroxide

  • Joseph Kanner
  • Stella Harel
  • Avior Menahem Salan
Part of the Basic Life Sciences book series (BLSC, volume 49)


Previous evidence for hydroxyl radical generation in ferric ion-derived Fenton reactions was presented by Walling et al.1 and Gutteridge2. Methemoglobin (MetHb) and metmyoglobin (MetMb), the ferric states of these proteins, are activated by H2O2 producing a short-lived intermediate composed of one oxidizing equivalent on the heme, forming a ferryl, and one on the globin, i.e. giving an oxo-ferryl hemeglobin radical3. Studies on metmyoglobin-peroxide show that although it is not identical with compounds I or II or horseradish peroxidase (HRP), it has some structural features in common with both4.


Fenton Reaction Free Iron Hydroxyl Radical Scavenger Membranal Lipid Peroxidation Hydroxyl Radical Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    C. Walling, Fenton!s reaction revisited, Acc. Chem. Res. 8:125 (1975).CrossRefGoogle Scholar
  2. 2.
    J. M. C. Gutteridge, Superoxide dismutase inhibits the superoxide-driven Fenton reaction at two different levels. Implications for a wider protective role, FEBS Lett. 185:Google Scholar
  3. 3.
    J. B. Fox, R. A. Nicholas, S. A. Ackerman, and C. E. Swift, A multiple wavelength analysis of the reaction between hydrogen peroxide and metmyoglobin. Biochemistry 13:5178 (1974).PubMedCrossRefGoogle Scholar
  4. 4.
    M. Chance, L. Powers, C. Kumar, and B. Chance, X-ray absorption studies of myoglobin peroxide reveal functional differences between globins and heme enzymes, Biochemistry 12:1259 (1986).CrossRefGoogle Scholar
  5. 5.
    S. M. H. Sadrzadeh, E. Graf, S. Scott Panter, P. E. Hollaway, and J. W. Eaton, Hemoglobin, biologic Fenton reagent. J. Biol. Chem. 259:14354 (1984).PubMedGoogle Scholar
  6. 6.
    J. M. C. Gutteridge, Iron promoters of the Fenton reaction and lipid peroxidation can be released from hemoglobin by peroxides, FEBS Lett. 201:291 (1986).PubMedCrossRefGoogle Scholar
  7. 7.
    M. E. Apgar and H. O. Hultin, Lipid peroxidation in fish muscle micro-somes in the frozen state, Cryobiology 19:154 (1982)PubMedCrossRefGoogle Scholar
  8. 8.
    J. Kanner and S. Harel, Initiation of membranal lipid peroxidation by activated metmyoglobin and methemoglobin, Arch. Biochem. Biophys. 237:314 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    J. M. C. Gutteridge, Reactivity of hydroxyl and hydroxyl-like radicals discriminated by release of thiobarbituric acid-reactive material from deoxysugars nucleosides and benzoate, Biochem. J. 224:761 (1984).PubMedGoogle Scholar
  10. 10.
    C. C. Winterbourn, The ability of scavengers to distinguish HO-production in the iron-catalyzed Haber-Weiss reaction: Composition of four assays for HO-, Free Rad. Biol. Med. 3:33 (1987).PubMedCrossRefGoogle Scholar
  11. 11.
    G. D. Lawrence, Ethylene formation from methionine and its analogs. in: “Handbook of Methods for Oxygen Radical Research,” R.A. Greenwald, ed, CRC Press Inc., Boca Raton, Florida (1985).Google Scholar
  12. 12.
    E. Graf, J. R. Mahoney, R. G. Bryant, and J. W. Eaton, Iron catalyzed hydroxyl radical formation. Stringent requirements for free iron coordination site, J. Biol. Chem. 259:3620 (1984).PubMedGoogle Scholar
  13. 13.
    P. L. Vandewalle and N. O. Petersen, Oxidation of reduced cytochrome C by hydrogen peroxide. FEBS Lett. 210:195 (1987).PubMedCrossRefGoogle Scholar
  14. 14.
    A. L. Tappel, Oxidative fat rancidity in food products. I. Linoleate oxidation catalyzed by hemin, hemoglobin and cytochrome C, Food Res. 18:560 (1953).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Joseph Kanner
    • 1
  • Stella Harel
    • 1
  • Avior Menahem Salan
    • 1
  1. 1.Department of ScienceVolcani CenterBet DaganIsrael

Personalised recommendations