Corneal Oxygen Scavenging Systems: Lysis of Corneal Epithelial Cells by Superoxide Anions

  • Rosalie Crouch
  • Zhu Ling
  • Betty J. Hayden
Part of the Basic Life Sciences book series (BLSC, volume 49)


The cornea is an unique tissue because it is exposed to insults from the external environment and the toxic effects of topically applied agents as well as the normal spectrum of physiological processes. Scarring can result from infection, trauma or numerous other causes which can compromise visual acuity. The cornea consists of three main layers: the epithelium, the stroma and the endothelium. Injury to either the epithelium or endothelium can lead to disruption of the underlying nonregenerative stroma resulting in permanent scarring.


Glutathione Peroxidase Corneal Epithelium Corneal Epithelial Cell South Carolina 29425 Human Corneal Epithelial Cell 
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  1. 1.
    I. Fridovich, The biology of oxygen radicals, Science 201:875 (1978).CrossRefPubMedGoogle Scholar
  2. 2.
    T. M. Redmond, E. J. Duke, W. H. Coles, J. A. V. Simson and R. K. Crouch, Localization of corneal superoxide dismutase by biochemical and histocytochemical techniques, Exp. Eye Res. 38:369 (1984).CrossRefPubMedGoogle Scholar
  3. 3.
    C. Beauchamp and I. Fridovich, Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels, Anal. Biochem. 44:276 (1971).CrossRefPubMedGoogle Scholar
  4. 4.
    A. Clairborne, Catalase activity, in: “CRC Handbook of Methods for Oxygen Radical Research,” R.A. Greenwald, ed., Boca Raton, FL, p. 283, (1985).Google Scholar
  5. 5.
    W. A. Gunzler, H. Kremers and L. Flohe, An improved coupled test procedure for glutathione peroxidase in blood, Z. Klin. Chem. Klin. Biochem. 12:444 (1974).PubMedGoogle Scholar
  6. 6.
    M. M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72:248 (1976).CrossRefPubMedGoogle Scholar
  7. 7.
    K. C. Bhuyan and D. K. Bhuyan, Regulation of hydrogen peroxide in eye humors. Effects of 3-araino-1H-1,2,4-triazole on catalase and glutathione peroxidase of rabbit eye. Biochim. Biophy. Acta, 497:641 (1977).CrossRefGoogle Scholar
  8. 8.
    U. Mayer, Comparative investigations of catalase activity in different ocular tissues in cattle and man, Albrecht von Graefes Arch. Klin. Ophthal. 213:261 (1980).Google Scholar
  9. 9.
    R. Crouch, J. Patrick, J. Gossey, and W.H. Coles, The effect of aging corneal and lens superoxide dismutase, Curr. Eye Res. 3:1119 (1984).CrossRefPubMedGoogle Scholar
  10. 10.
    K.T. Brunner, J. Mauel, J.C. Cerrottini, and B. Chapris, Quantitative assay of the lytic action of immune lymphoid cells on 51Cr labeled target cells in vitro: Inhibition by isoantibody and by drugs, Immunol. 14:181 (1968).Google Scholar
  11. 11.
    R. Neville, P. Dennis, D. Sens, and R.K. Crouch, Preservative cytotoxicity to cultured corneal epithelial cells, Curr. Eye Res. 3:1119 (1984).CrossRefGoogle Scholar
  12. 12.
    D. Darr and I. Fridovich, Irreversible inactivation of catalase by 3-amino-1,2,4-triazole, Biochem. Pharmacol. 35:36 (1986).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Rosalie Crouch
    • 1
  • Zhu Ling
    • 1
  • Betty J. Hayden
    • 1
  1. 1.Department of OphthalmologyMedical University of South CarolinaCharlestonUSA

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