Anterior Chamber Hydrogen Peroxide: Effects of 3-Aminotriazole on Peroxide Kinetics and on the Status of Glutathione

  • Stephen Csukas
  • Anastasios Costarides
  • Michael V. Riley
  • Keith Green
Part of the Basic Life Sciences book series (BLSC, volume 49)


Previous studies using in vitro methods have shown that the anterior segment tissues, particularly the corneal endothelium, have the capacity to handle excess superoxide anion, but the capability to detoxify hydrogen peroxide can be exceeded. The data indicated that, of all the phototoxic by-products, hydrogen peroxide was the compound capable of inducing the most damage in the anterior segment of the eye.1–3


Catalase Activity Anterior Chamber Aqueous Humor Anterior Segment Ocular Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    D.S. Hull, S. Csukas, K. Green, and V. Livingston. Hydrogen peroxide and corneal endothelium. Acta Ophthalmol. 59:409 (1981).Google Scholar
  2. 2.
    D.S. Hull, K. Green, L. Thomas, and N. Alderman. Hydrogen peroxide mediated corneal endothelial damage: Induction by oxygen free radical. Invest. Ophthalmol. Vis. Sci. 25:1246 (1984).PubMedGoogle Scholar
  3. 3.
    D.S. Hull, K. Green, and R.D. Elijah. Effect of oxygen free radical products on iris vascular permeability. Acta Ophthalmol. 63:513 (1985).Google Scholar
  4. 4.
    S. Csukas and K. Green. Effects of intracameral hydrogen peroxide in the rabbit anterior chamber. Invest. Ophthalmol. Vis. Sci. 29:335 (1988).PubMedGoogle Scholar
  5. 5.
    K.C. Bhuyan and D.K. Bhuyan. Regulation of hydrogen peroxide in eye humors. Effect of 3-amino-1H-1,2,4-triazole on catalase and glutathione peroxidase of rabbit eye. Biochim. Biophys. Acta 497:641 (1977).PubMedCrossRefGoogle Scholar
  6. 6.
    A. Pirie. Glutathione peroxidase in lens as a cause of hydrogen peroxide in aqueous humor. Biochem. J. 96:244 (1965).PubMedGoogle Scholar
  7. 7.
    M.C. Ng and M.V. Riley. Relation of intracellular levels and redox state of glutathione to endothelial function in the rabbit cornea. Exp. Eye Res. 30:511 (1980).PubMedCrossRefGoogle Scholar
  8. 8.
    A. Costarides, D. Birnbaum, S. Csukas, E. Forbes, and K. Green. Morphological sequelae of anterior segment hydrogen peroxide in young and adult rabbits with or without 3-aminotriazole treatment, in: “Oxygen Radicals in Biology and Medicine,” M. Simic, J. Ward and K. Taylor, eds., Plenum Press, New York (1988).Google Scholar
  9. 9.
    O. Hockwin and C. Ohrloff. The eye in the elderly: Lens, in: “Geriatrics 3,” D. Platt, ed., Springer-Verlag, Berlin, Pp. 373 (1984).CrossRefGoogle Scholar
  10. 10.
    A. Spector and W.H. Garner. Hydrogen peroxide and human cataracts. Exp. Eye. Res. 33:673 (1981).PubMedCrossRefGoogle Scholar
  11. 11.
    S. Csukas, A. Costarides, M.V. Riley, and K. Green. Hydrogen peroxide in the rabbit anterior chamber: Effect on glutathione, and catalase effects on peroxide kinetics. Curr. Eye Res. 6:1395 (1987).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Stephen Csukas
    • 1
    • 2
  • Anastasios Costarides
    • 1
    • 2
  • Michael V. Riley
    • 3
  • Keith Green
    • 1
    • 2
  1. 1.Department of OphthalmologyMedical College of GeorgiaAugustaUSA
  2. 2.Department of Physiology and EndocrinologyMedical College of GeorgiaAugustaUSA
  3. 3.Eye Research InstituteOakland UniversityRochesterUSA

Personalised recommendations