Potential of Methylene Blue to Block Oxygen Radical Generation in Reperfusion Injury

  • Michael J. Kelner
  • Richard  Bagnell
  • Braden Hale
  • Nicholas M. Alexander
Part of the Basic Life Sciences book series (BLSC, volume 49)


Superoxide and hydroxyl radical generation have been implicated in a variety of pathological conditions, including ischemic injury in myocardial, renal, and skin-flap tissue, as reperfusion of the ischemic tissue presumably leads to a burst of free radical generation 1–4,14. Tissue hypoxia during ischemia results in the conversion of xanthine dehydrogenase to xanthine oxidase and concomitant breakdown of ATP to hypoxanthine 14. Upon reperfusion the xanthine oxidase oxidizes the hypoxanthine, and large quantities of superoxide, hydrogen peroxide, and possibly hydroxyl radicals are produced. Previous attempts to inhibit oxygen radical toxicity have focused on removing the superoxide or hydroxyl radicals after their production.


Uric Acid Methylene Blue Xanthine Oxidase Molybdenum Disulfide Flavin Adenine Dinucleotide 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Michael J. Kelner
    • 1
  • Richard  Bagnell
    • 1
  • Braden Hale
    • 1
  • Nicholas M. Alexander
    • 1
  1. 1.Division of Laboratory Medicine, Department of Pathology, San Diego Medical CenterUniversity of CaliforniaSan DiegoUSA

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