Cellular Targets of H2O2 and HOCl Induced Injury
During the respiratory burst stimulated neutrophils or macrophages release cell damaging concentrations of oxidants in any area of inflammation.1–3 In order to evaluate the role of various oxidants in target cell injury, we have studied the primary targets of oxidants in cells and the biochemical pathways responsible for cellular damage. The target cells used were P388 Dl murine macrophage-like tumor cells, GM 1380 human embryo lung fibroblasts, human peripheral lymphocytes and rabbit alveolar macrophages. The oxidants used were H2O2, HOCl and phorbol myristate acetate (PMA)-stimulated PMNs or neuiroplasts — enucleated PMNs that still undergo the respiratory burst.
KeywordsElectron Spin Resonance Phorbol Myristate Acetate Adult Respiratory Distress Syndrome Phorbol Myristate Acetate Hexose Monophosphate
List of Abbreviations
phorbol myristate acetate
DNA strand breaks
hexose monophosphate shunt
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