Vanadate Stimulation of Pyridine Nucleotide Oxidation in Mammalian Liver Microsomal Membranes
Vanadate has been shown in numerous experiments to markedly stimulate the oxidation of NAD(P)H in biological membranes. However, explanations of the nature of this stimulation often have been contradictory. Vanadate-catalyzed oxidations have been postulated to be a result of a specific membrane-associated NADH oxidase.1 Liochev and Fridovich2 have reported that this phenomenon reflects a vanadate stimulation of NAD(P)H oxidation by superoxide rather than by vanadate-stimulated NAD(P)H oxidases or dehydrogenases. The purpose of the present study was to characterize the nature of the vanadate stimulation of pyridine nucleotide oxidation in membranes from rat liver and to determine if vanadate is capable of stimulating NADPH oxidation in systems containing purified NADPH cytochrome P-450 reductase.
KeywordsOxidase Activity Pyridine Nucleotide NADH Oxidase Sodium Molybdate NADPH Oxidation
Unable to display preview. Download preview PDF.