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Vanadate Stimulation of Pyridine Nucleotide Oxidation in Mammalian Liver Microsomal Membranes

  • Roger A. CoulombeJr.
  • David W. Reif
  • Randal J. Keller
  • Donald P. Briskin
  • Steven D. Aust
  • Raghubir P. Sharma
Part of the Basic Life Sciences book series (BLSC, volume 49)

Abstract

Vanadate has been shown in numerous experiments to markedly stimulate the oxidation of NAD(P)H in biological membranes. However, explanations of the nature of this stimulation often have been contradictory. Vanadate-catalyzed oxidations have been postulated to be a result of a specific membrane-associated NADH oxidase.1 Liochev and Fridovich2 have reported that this phenomenon reflects a vanadate stimulation of NAD(P)H oxidation by superoxide rather than by vanadate-stimulated NAD(P)H oxidases or dehydrogenases. The purpose of the present study was to characterize the nature of the vanadate stimulation of pyridine nucleotide oxidation in membranes from rat liver and to determine if vanadate is capable of stimulating NADPH oxidation in systems containing purified NADPH cytochrome P-450 reductase.

Keywords

Oxidase Activity Pyridine Nucleotide NADH Oxidase Sodium Molybdate NADPH Oxidation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    S. Vijaya, F.L. Crane, and T. Ramasarma, A vanadate-stimulated NADH oxidase in erythrocyte membranes generates hydrogen peroxide, Mol. Cell. Biochem. 62:175 (1984).PubMedCrossRefGoogle Scholar
  2. 2.
    S. Liochev and I. Fridovich, The vanadate-stimulated oxidation of NAD(P)H by biomembranes is a superoxide-initiated free radical chain reaction, Arch. Biochem. Biophvs. 250:139 (1986).CrossRefGoogle Scholar
  3. 3.
    R. A. Coulombe, D.P. Briskin, R.J. Keller, W.R. Thornley, and R.P. Sharma, Vanadate-dependent oxidation of pyridine nucleotides in rat liver microsomal membranes, Arch. Biochem. Biophys. 255:267 (1987).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Roger A. CoulombeJr.
    • 1
  • David W. Reif
    • 1
  • Randal J. Keller
    • 1
  • Donald P. Briskin
    • 2
  • Steven D. Aust
    • 1
  • Raghubir P. Sharma
    • 1
  1. 1.Graduate Programs in Toxicology and Molecular Biology and BiochemistryUtah State UniversityLoganUSA
  2. 2.Department of AgronomyUniversity of IllinoisUrbanaUSA

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