Reduction of 5-Nitroimidazoles, Nitrofurazone, and 2,4-Dinitrophenol to their Free Radical Metabolites by Tritrichomonas Foetus Hydrogenosomes
Oxidative decarboxylation of pyruvate is catalyzed in trichomonads by pyruvate:ferredoxin oxidoreductase (PFO). This enzyme is localized in characteristic membrane-bound organelles that are termed hydrogenosomes on the basis of their biochemical characteristics. The main metabolic function of hydrogenosomes is the conversion of pyruvate to acetate via acetyl-CoA, accompanied by substrate level phosphorylation coupled to production of molecular hydrogen (Figure 1).
KeywordsElectron Spin Resonance Redox Cycling Spin Adduct Substrate Level Phosphorylation Thiamine Diphosphate
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