Thiyl Free Radical Metabolites of Thiol Drugs and Glutathione
Lactoperoxidase, a prototypical mammalian peroxidase, is part of an antimicrobial system found in secreted fluids.1 It is believed to act by oxidation of thiocyanate.2 Not surprisingly, it will also metabolize thiol moieties, oxidizing them to free radical intermediates. Thiol compounds are important in pharmacology and toxicology, and we have examined the production of highly reactive thiyl free radicals by this mechanism.
KeywordsAmes Test North Carolina 27514 Thiol Compound Instrumental Condition Thiyl Radical
Unable to display preview. Download preview PDF.
- 3.P.C. Jocelyn, in: “Biochemistry of the SH Group,” Academic Press, New York (1972).Google Scholar
- 6.J. Peisach and W.E. Blumberg, A mechanism for the action of penicillamine in the treatment of Wilson’s disease, Mol. Pharm. 5: 200 (1969).Google Scholar
- 8.J.B. Gerber, J.S. Macdonald, R.D. Harbison, J.-P. Villeneuve, A.J.J. Wood, and A.S. Nies, Effect of N-acetylcysteine on hepatic covalent binding of paracetamol, Lancet i: 657 (1977).Google Scholar
- 10.C. Mottley, K. Toy, and R.P. Mason, Oxidation of thiol drugs and biochemicals by the lactoperoxidase/hydrogen peroxide system, Mol. Pharm. 31:417 (1987).Google Scholar