Nuclear Magnetic Resonance (NMR) Spectroscopy: Basic Principles and Some Applications to Studies of Cerebral Metabolism

  • J. Feeney
Part of the NATO ASI Series book series (NSSA, volume 153)


Over the last 20 years there has been widespread application of NMR to biological problems. Initially much of the work was on isolated biological molecules and was concerned with studies of molecular structures and conformations, dynamics and interactions of complex biological molecules such as proteins, nucleic acids, phospholipids and oligosaccharides. In 1973 two developments occurred which considerably extended the range of application of NMR to biological studies. In the first it was shown that it was possible to measure 31P NMR spectra of metabolites in cells and intact tissues (Moon and Richards1 and Moult et al.2) and these early experiments stimulated the use of NMR for ‘in vivo’ studies which have now been extended to investigations of intact organs in human subjects. The second important advance was the demonstration by Lauterbur3 and Mansfield and Grannell4 that NMR could be used as a proton density imaging technique further development of which resulted in the present day availability of commercial NMR clinical imaging systems and provided the basis for the blood flow studies described later in this text.


Nuclear Magnetic Resonance Nuclear Magnetic Resonance Spectrum Nuclear Magnetic Resonance Spectroscopy Free Induction Decay Larmor Frequency 
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  1. 1.
    R.B. Moon and J.H. Richards, J. Biol. Chem., 248: 7276 (1973).PubMedGoogle Scholar
  2. 2.
    D.I. Moult, S.J.W. Busby, D.G. Gadian, G.K. Radda, R.E. Richards and P.J. Seeley, Nature (London) 252: 285 (1974).CrossRefGoogle Scholar
  3. 3.
    P.C. Lauterbur, Nature (London) 242: 190 (1973).CrossRefGoogle Scholar
  4. 4.
    P. Mansfield and P.K. Grannell, J. Phys. Chem. 6: L422 (1973).Google Scholar
  5. 5.
    J.W. Emsley, J. Feeney and L.H. Sutcliffe, “High Resolution Nuclear Magnetic Resonance Spectroscopy”, Vols. I and II Pergamon Press (1965).Google Scholar
  6. 6.
    O. Jardetzky and G.C.K. Roberts, “NMR in Molecular Biology”, Academic Press, New York (1981).Google Scholar
  7. 7.
    D.G. Gadian, “Nuclear Magnetic Resonance and its Applications to Living Systems”, Clarendon Press, Oxford (1982).Google Scholar
  8. 8.
    H.S. Bachelard, D.W.G. Cox, J. Feeney and P.G. Morris, Biochem. Soc. Trans., 13: 835 (1986).Google Scholar
  9. 9.
    K. Thurlborn, G.H. du Boulay, L.W. Duchen and G.S. Radda, J. Cerebral Blood Flow, 2: 299 (1982).CrossRefGoogle Scholar
  10. 10.
    J.W. Prichard, J.R. Alger, K.L. Behar, O.A.C. Petroff and R.G. Shulman, Proc. Natl. Acad. Sci. USA, 80: 2748 (1983).CrossRefGoogle Scholar
  11. 11.
    E.B. Cady, M.J. Dawson, P.L. Hope, P.S. Tofts, A.M. de L. Costello, D.T. Delpy, E.O.R. Reynolds and D.R. Wilkie, Lancet 1: 1059 (1983).PubMedCrossRefGoogle Scholar
  12. 12.
    M.J. Avison, H.P. Hetherington and R.G. Shulman, “Applications of NMR to Studies of Tissue Metabolism”, Ann. Rev. Biophys. and Biophys. Chem., 15: 377 (1986).CrossRefGoogle Scholar
  13. 13.
    K.L. Behar, J.A. den Hollander, M.E. Stromski, T. Ogino, R.G. Shulman, O.A.C. Petroff and J.W. Prichard, Proc. Natl. Acad. Sci., USA 80: 4945 (1983).CrossRefGoogle Scholar
  14. 14.
    K.L. Behar, D.L. Rothman, R.G. Shulman, O.A.C. Petroff and J.W. Prichard, Proc. Natl. Acad. Sci. USA 81: 2517 (1984).CrossRefGoogle Scholar
  15. 15.
    B. Chance, Y. Nakase, M. Bond, J.S. Leigh Jr. and G. McDonald, Proc. Natl. Acad. Sci. USA 75: 4925 (1978).CrossRefGoogle Scholar
  16. 16.
    D.L. Rothman, K.L. Behar, H.P. Hetherington, J.A. van Hollander, M.R. Bendall, 0.A.C. Petroff and R.G. Shulman, Proc. Natl. Acad. Sci. USA 82: 1633 (1985).CrossRefGoogle Scholar
  17. 17.
    D.W.G. Cox, P.G. Morris, J. Feeney and H.S. Bachelard, Biochem. J. 212: 365 (1983).PubMedGoogle Scholar
  18. 18.
    0.A.C. Petroff, J.W. Prichard, K.L. Behar, J.R. Alger, J.A. den Hollander and R.G. Shulman, Neurology 35: 781 (1985).PubMedCrossRefGoogle Scholar
  19. 19.
    E.A. Shoubridge, R.W. Briggs and G.K. Radda, FEBS Lett. 140: 288 (1982).CrossRefGoogle Scholar
  20. 20.
    K. Brindle, Prog. Nucl. Mag. Spectros. In press.Google Scholar
  21. 21.
    R.S. Balaban, D.G. Gadian and G.K. Radda, Kidney Int., 20: 575 (1981).PubMedCrossRefGoogle Scholar
  22. 22.
    P.G. Morris, J. Feeney, D.W.G. Cox and H.S. Bachelard, Biochem. J., 212: 777 (1985).Google Scholar
  23. 23.
    0.A.C. Petroff, J.W. Prichard, T. Ogino, M.J. Avison, J.R. Alger and R.G. Shulman, Ann. Neurol. In press (1986).Google Scholar
  24. 24.
    M. Hilberman, V.H. Subramanian, J. Haslegrove, J.B. Cone, J.W. Egan, L. Gyulai and B. Chance, J. Cereb. Blood Flow Metab. 4: 366 (1984).CrossRefGoogle Scholar
  25. 25.
    L. Gyulai, L. Bolinger, J.S. Leigh, C. Barlow and B. Chance, FEBS Lett. 178 (1): 137 (1984).PubMedCrossRefGoogle Scholar
  26. 26.
    D.G. Gadian, R.S.J. Frackowiak, H.A. Crockard, E. Proctor, K. Allen, S.R. Williams and R.W.R. Russell, J. Cereb. Blood Flow Metab. In press.Google Scholar
  27. 27.
    H.A. Crockard, D.G. Gadian, R.S.J. Frackowiak, E. Proctor, K. Allen, S.R. Williams and R.W.R. Russell, J. Cereb. Blood Flow Metab. In press.Google Scholar
  28. 28.
    K.L. Behar, J.A. den Hollander, 0.A.C. Petroff, H.P. Hetherington, J.W. Prichard and R.G. Shulman, J. Neurochem., 44: 1045 (1985).PubMedCrossRefGoogle Scholar
  29. 29.
    S.J. Kopp, J. Krieglstein, A. Freidank, A. Rachman, A. Siebert and S.M. Cohen, J. Neurochem. 43: 1716 (1984).PubMedCrossRefGoogle Scholar
  30. 30.
    R.R. Ernst and W.A. Anderson, Rev. Sci. Instruments, 37: 93 (1966).CrossRefGoogle Scholar
  31. 31.
    J.W. Cooper, “Topics in Carbon-13C NMR Spectroscopy” Vol. 2: 406 (1976) Editor G.C. Levy, John Wiley and Sons.Google Scholar
  32. 32.
    P.L. Hope, E.B. Cady, P.S. Tofts, P.H. Hamilton, A.M. Costello, Lancet, 2: 366 (1984).PubMedCrossRefGoogle Scholar
  33. 33.
    K.L. Behar, Ph.D. Thesis. Yale University (1985) — results discussed in Reference 14.Google Scholar
  34. 34.
    D. Shaw, “Fourier Transform NMR”, Elsevier, Amsterdam (1976).Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • J. Feeney
    • 1
  1. 1.MRC Biomedical NMR CentreNational Institute for Medical Research, Mill HillLondonEngland

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