Detection of Free Radicals in Cerebral Tissue and their Relation to Cerebral Hypoxia/Ischemia

  • Shigeki Imaizumi
  • Teiji Tominaga
  • Hiroshi Uenohara
  • Hiroyuki Kinouchi
  • Takashi Yoshimoto
  • Jiro Suzuki
Part of the Advances in Behavioral Biology book series (ABBI, volume 35)


In this paper, the possibility that cerebral ischemia or cerebral hypoxia may initiate a series of free radical reactions in brain lipid constituents was explored. Though chemiluminescence (CL) detection and E.S.R. (electron spin resonance) measurement are indeed useful for determination of whole cellular component autoxidation, they are not sufficient to verify free radical mediated “lipid” peroxidation. Therefore we needed the study of cellular membrane lipid metabolism in the ischemic brain.

High luminous value of CL and BPN (N-tert-Butyl-α-phenylnitrone) trapped radical in ESR (AN=16.2−16.5G and A β H =3.6−3.8G) were obtained in the ischemic brain especially during recirculation.

At the onset of ischemia, PIP2 and PIP rapidly decreased and DG increased symmetrically and in recirculation they tended to return to preischemic levels. In FFAs accumulated throughout ischemia, C18.0 and C20.4 increased rapidly during early ischemia by deacylation of DG. During recirculation unsaturated FFAs of the type C20:4 were rapidly reduced probably due to attack of free radicals.

Differences between hypoxia and ischemia were explored by measurement of energy state and CL. To clarify the mitochondrial electron flow in ischemia/hypoxia, ESR study at 77K was applied.


Electron Spin Resonance Ischemic Brain Electron Spin Resonance Signal Hypoxic Brain Inositol Triphosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.









free fatty acid


inositol triphosphate


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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Shigeki Imaizumi
    • 1
  • Teiji Tominaga
    • 1
  • Hiroshi Uenohara
    • 1
  • Hiroyuki Kinouchi
    • 1
  • Takashi Yoshimoto
    • 1
  • Jiro Suzuki
    • 1
  1. 1.Division of Neurosurgery, Institute of Brain DiseasesTohoku University, School of MedicineSendaiJapan

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