Advertisement

Ischemic Damage of Rat Hippocampus and Basal Ganglia: Light Microscopical and Biochemical Changes

  • Rainald Schmidt-Kastner
  • Wulf Paschen
  • Janus Szymas
  • Konstantin-Alexander Hossmann
Part of the Advances in Behavioral Biology book series (ABBI, volume 35)

Summary

Rats were subjected to 30 min severe forebrain ischemia using a modified four-vessel occlusion (4VO) model. Biochemical determinations and measurements of regional cerebral blood flow revealed injury of hippocampus and striatum. Morphological analysis showed a pattern of selective vulnerability in both structures. Concomitant immunohistochemical investigations revealed massive glial proliferation and extravasation of serum-albumin.

Keywords

Mossy Fiber Ischemic Damage Forebrain Ischemia Transient Cerebral Ischemia Selective Vulnerability 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Block, G.A., and Pulsinelli, W.A., 1987, Excitatory amino acid receptor antagonists: Failure to prevent ischemic neuronal damage. J. Cereb. Blood Flow Metabol. 7, Suppl. 1: 5149.Google Scholar
  2. Chang, H.T., Wilson, C.J., and Kitai, S.T., 1982, A Golgi study of rat neostriatal neurons: light microscopic analysis, J. Comp. Neurol., 208: 107–126.CrossRefGoogle Scholar
  3. Coyle, P., 1976, Vascular patterns of the rat hippocampal formation. Exp. Neurol., 52: 447–458.CrossRefGoogle Scholar
  4. Csiba, L., Paschen, W., and Hossmann, K.-A., 1983, A topographpic quantitative method for measuring brain tissue pH under physiological and pathophysiological conditions. Brain Res., 289: 334–337CrossRefGoogle Scholar
  5. Cuello, A.C., Priestley, J.V.P., and Paxinos, G., 1985, Substance-P and enkephalin containing pathways, in: “The Rat Nervous System”, Vol. 1, ed. G. Paxinos, Academic Press, Sydney, pp. 441–469Google Scholar
  6. Francis, A., and Pulsinelli, W.A., 1982, The response of GABAergic and cholinergic neurons to transient cerebral ischemia. Brain Res., 243: 271–278.CrossRefGoogle Scholar
  7. Franck, J.E., 1984, Dynamic alterations in hippocampal morphology following intra-ventricular kainic acid, Acta Neuropathol., 62: 242–253.CrossRefGoogle Scholar
  8. Graybiel, A.M., and Ragsdale, C.W. Jr., 1983, Biochemical anatomy of the striatum, in: “Chemical Neuroanatomy”, ed. P.C. Emson, Raven Press, New York, pp. 427–504.Google Scholar
  9. Hallmayer, J., Hossmann, K.-A., and Mies, G., 1985, Low dose of barbiturates for prevention of hippocampal lesions after brief ischemic periods, Acta Neuropathol., 68: 27–31CrossRefGoogle Scholar
  10. Hedreen, J.C., Bacon, S.J., and Price, D.L., 1985, A modified histochemical technique to visualize acetylcholinesterase-containing axons, J. Histochem. Cytochem., 33: 134–140CrossRefGoogle Scholar
  11. Johansen, F.F., J$rgensen, M.B., and Diemer, N.H., 1983, Resistance of hippocampal CA-1 interneurons to 20 min of transient cerebral ischemia in the rat. Acta Neuropathol., 61: 135–140.CrossRefGoogle Scholar
  12. Johansen, F.F., Jgrgensen, M.B., and Diemer, N.H., 1986, Ischemic CA-1 pyramidal cell loss is prevented by preischemic colchicine destruction of dentate gyrus granule cells, Brain Res., 377: 344–347.CrossRefGoogle Scholar
  13. Johansen, F.F., Zimmer, J., and Diemer, N.H., 1987, Early loss of somato-statin neurons in dentate hilus after cerebral ischemia in the rat precedes CA-1 pyramidal cell loss, Acta Neuropathol., 73: 110–114.CrossRefGoogle Scholar
  14. Jrgensen, M.B., and Diemer, N.H., 1982, Selective neuron loss after cerebral ischemia in the rat: possible role of transmitter glutamate, Acta neurol. scandinay., 66: 536–546.CrossRefGoogle Scholar
  15. Jsrgensen, M.B., Johansen, F.F., and Diemer, N.H., 1987, Removal of the entorhinal cortex protects hippocampal CA-1 neurons from ischemic damage. Acta Neuropathol., 73: 189–194.CrossRefGoogle Scholar
  16. Joyce, J.N., Loeschen, S.K., and Marshall, J.F., 1985, Dopamine D-2 receptors in rat caudate-putamen: the lateral to medial gradient does not correspond to dopaminergic innervation, Brain Res. 338: 209–218.CrossRefGoogle Scholar
  17. Kirino, T., Tamura, A., and Sano, K., 1984, Delayed neuronal death in the rat hippocampus following transient forebrain ischemia, Acta Neuropathol., 64: 139–147.CrossRefGoogle Scholar
  18. Kogure, K., and Alonso, O.F., 1978, A pictorial representation of endogenous brain ATP by a bioluminescent method. Brain Res., 154: 273–284.CrossRefGoogle Scholar
  19. Kubota, Y., Inagaki, S., Shimada, S., Kito, S., Eckenstein, F., and Tohymama, 1987, Neostriatal cholinergie neurons receive direct synaptic inputs from dopaminergic neurons, Brain Res., 413: 179–184.CrossRefGoogle Scholar
  20. Lowry, O.H., and Passoneau, J.V., 1972, “A Flexible System of Enzymatic Analysis”, Academic Press; New York, London.Google Scholar
  21. Lynch, G.S., Lucas, P.A., and Deadwyler, S.A., 1972, The demonstration of acetylcholinesterase containing neurones within the caudate nucleus of the rat, Brain Res., 45: 617–621.CrossRefGoogle Scholar
  22. Meyer, A., 1936, The selective regional vulnerability of the brain and its relation to psychiatric problems, Proc. Royal Soc. Med., 29: 1175–1181.Google Scholar
  23. Pellegrino, L.J., Pellegrino, A.S., and Cushman, A.J., 1979, “A stereo- taxic atlas of the rat brain”, Plenum Press, New York, London.Google Scholar
  24. Peters, S., Koh, J., and Choi, D.W., 1987, Zinc selectively blocks the action of N-methyl-D-aspartate on cortical neurons, Science, 236: 589–593.CrossRefGoogle Scholar
  25. Petito, C.K., and Pulsinelli, W.A., 1984, Delayed neuronal recivery and neuronal death in rat hippocampus following severe cerebral ischemia: possible relationship to abnormalities in neuronal processes. J. Cereb. Blood Flow Metabol., 4: 194–205.CrossRefGoogle Scholar
  26. Pontén, U., Ratcheson, R.A., Salford, L.G., and Siesjö, B.K., 1973, Optimal freezing conditions for cerebral metabolites in rats, J. Neurochem., 21: 1127–1138.CrossRefGoogle Scholar
  27. Pulsinelli, W.A., and Brierley, J.B., 1979, A new model of bilateral hemispheric ischemia in the unanesthetized rat, Stroke, 10: 267–272.CrossRefGoogle Scholar
  28. Pulsinelli, W.A., Brierley, J.B., and Plum, F., 1982. Temporal profile of neuronal damage in a model of transient forebrain ischemia, Ann. Neurol., 11: 491–498.Google Scholar
  29. Pulsinellí, W.A., and Duffy, T.E., 1983, Regional energy balance in rat brain after transient forebrain ischemia. J. Neurochem., 40: 1500–1503.CrossRefGoogle Scholar
  30. Rieke, G.K., Bowers, D.E., and Penn, P., 1981, Vascular supply pattern to rat caudatoputamen and globus pallidus: scanning electron-microscopic study of vascular endocasts of stroke-prone vessels. Stroke, 12: 840–847.CrossRefGoogle Scholar
  31. Schmidt-Kastner, R., Paschen, W., Ueki, M., and Hossmann, K.-A., 1987, Ischemic damage of the basal ganglia after four-vessel occlusion in rat: morphological and regional biochemical investigation. J. Cereb. Blood Flow Metabol., 7,Supp1. 1: S139.Google Scholar
  32. Simon, R.P., Swan, J.H., Griffith, T., and Meldrum, B.S., 1984, Blockade of N-methyl-D-aspartate receptors may protect against ischemic damage in the brain, Science, 226: 850–852.CrossRefGoogle Scholar
  33. Sloviter, R.S., 1982, A simplified Timm stain procedure compatible with formaldehyde fixation and routine paraffin embedding of rat brain, Brain Res. Bull., 8: 771–774.CrossRefGoogle Scholar
  34. Smith, M.-L., Auer, R.N., and Siesjö, B.K., 1984, The density and distribution of ischemic brain injury in the rat following 2–10 min of forebrain ischemia, Acta Neuropathol., 64: 319–332.CrossRefGoogle Scholar
  35. Walaas, I., 1983, The hippocampus, in: “Chemical Neuroanatomy”, P.C. Emson, ed., Raven Press, New York, pp. 337–358.Google Scholar
  36. Wieloch, T., 1985, Neurochemical correlates to selective neuronal vulnerability, Prog. Brair Res., 63: 69–85.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Rainald Schmidt-Kastner
    • 1
  • Wulf Paschen
    • 1
  • Janus Szymas
    • 1
  • Konstantin-Alexander Hossmann
    • 1
  1. 1.Abteilung für experimentelle NeurologieMax-Planck-Institut für Neurologische ForschungKöln 91 (Merheim)Germany

Personalised recommendations