Structure and Function of the En/Spm Transposable Element System of ZeaMays: Identification of the Suppressor Component of En
The transposable element system Enhancer (En) of Zea mays was originally identified by Peterson (12) at the pale green locus as a mutable allele. Subsequently, the control of this mutability was shown to be homologous to the Suppressor-Mutator (Spm) system (5) both genetically (13) as well as molecularly (11). This system is comprised of two components, one of which (En, Spm) is capable of autonomous transposition and encodes all functions associated with this system. The second component, the nonautonomous Inhibitor (I) (12), is unable to promote transposition but can be trans-activated to transpose by an En/Spm element present in the same genome. Several I elements have been isolated (1,15–17,21,22) which bear the termini of En/Spm but carry internal deletions of varying extent.
KeywordsTransposable Element Terminal Inverted Repeat Internal Deletion Autonomous Element Cold Spring Harbor SymposIum
Unable to display preview. Download preview PDF.
- 4.Masson, P., R. Surosky, J.A. Kingsbury, and N.V. Fedoroff (1987) Genetic and molecular analysis of the Spm-dependent a-m2 alleles of the maize a locus. Genetics (in press).Google Scholar
- 5.McClintock, B. (1954) Mutations in maize and chromosomal aberrations in Neurospora. Carnegie Institute Washington Yearbook 53:254–260.Google Scholar
- 6.McClintock, B. (1963) Further studies of gene-control systems in maize. Carnegie Institute Washington Yearbook 62:486–493.Google Scholar
- 7.McClintock, B. (1965) The control of gene action in maize. Brookhaven Symposium on Biology 18:162–184.Google Scholar
- 8.McClintock, B. (1971) The contribution of one component of a control system to versatility of gene expression. Carnegie Institute Washington Yearbook 70:5–17.Google Scholar
- 12.Peterson, P.A. (1953) A mutable pale green locus in maize. Genetics 38:682–683.Google Scholar
- 14.Peterson, P.A. (1985) The isolation of En-1 in the wx-844 allele. Maize Genet. Coop. Newslett. 59:3.Google Scholar
- 15.Sommer, H., R. Hehl, E. Krebbers, R. Piotrowiak, W-E. Lönnig, and H. Saedler (1987) Transposable elements of Antirrhinum majus. (See this Volume.).Google Scholar
- 21.Tacke, E., Zs. Schwarz-Sommer, P.A. Peterson, and H. Saedler (1986) Molecular analysis of states of the A1 locus of Zea mays. Maydica 31:83–91.Google Scholar