Advertisement

Recombinant Mutable Alleles of the Maize R Gene

  • Jerry L. Kermicle
Part of the Basic Life Sciences book series (BLSC, volume 47)

Abstract

Three insertions of Dissociation (Ds) into an R allele that confers strong seed color (Sc) have been transferred by crossing over into a plant color allele (P). The recombinant products show partial to complete inhibition of plant pigmentation in the absence of Activator (Ac). The rank of pigmentation levels in the sheaths of seedling leaves and in anthers is the same for the (P) recombinants as for seed color in the original (Sc) mutables. There is one reversal of rank order in coleoptiles. In the presence of Ac, intensity of sectoring in anthers parallels that in seeds of the respective (Sc) counterparts. The recombinant (P) mutables originate from (Sc)Ds/(P) heterozygotes as a reciprocal product of crossing over that restores normal (Sc) phenotype. For r-m3, both products were recovered together following meiotic nondisjunction. The transfer of mutability to (P) and the restoration of (Sc) pigmentation were used as criteria to investigate the relationship of (Sc) and (P) with other R genic elements. Transfer and restoration experiments involving (Sc) and (P) have succeeded in every combination tested. Although functional (P) derivations were obtained from (P)m-1 heterozygotes involving the R elements (S) and (D), mutable forms of these elements were not obtained. Neither transfer nor restoration occurred when (P)m-1 was combined with R-cherry, indicating a third class of R allele organization.

Keywords

Transposable Element Flank Marker Seed Color Distal Marker Intragenic Recombination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bray, R.A., and R.A. Brink (1966) Mutation and paramutation at the R locus in maize. Genetics 54:137–149.PubMedGoogle Scholar
  2. 2.
    Brink, R.A. (1973) Paramutation. Ann. Rev. Genet. 7:129–152.PubMedCrossRefGoogle Scholar
  3. 3.
    Dooner, H.K. (1979) Identification of an R-locus region that controls the tissue specificity of anthocyanin formation in maize. Genetics 93:703–710.PubMedGoogle Scholar
  4. 4.
    Dooner, H.K. (1986) Genetic fine structure of the bronze locus in maize. Genetics 113:1021–1036.PubMedGoogle Scholar
  5. 5.
    Donner, H.K., and J.L. Kermicle (1986) The transposable element Ds affects the pattern of intragenic recombination at the bz and R loci in maize. Genetics 113:135–143.Google Scholar
  6. 6.
    Kermicle, J.L. (1970) Somatic and meiotic instability of R-stippled, an aleurone spotting factor in maize. Genetics 64:247–258.PubMedGoogle Scholar
  7. 7.
    Kermicle, J.L. (1970) Dependence of the R-mottled aleurone phenotype in maize on mode of sexual transmission. Genetics 66:69–85.PubMedGoogle Scholar
  8. 8.
    Kermicle, J.L. (1973) Organization of paramutational components of the R locus in maize. Brookhaven Symposium in Biology 25:262–280.Google Scholar
  9. 9.
    Kermicle, J.L. (1980) Probing the component structure of a maize gene with transposable elements. Science 208:1457–1459.PubMedCrossRefGoogle Scholar
  10. 10.
    Kermicle, J.L. (1985) Alternative tests of allelism. In Plant Genetics, M. Freeling, ed. Alan R. Liss, Inc., New York, pp. 491–507.Google Scholar
  11. 11.
    Nelson, O.E. (1986) The waxy locus in maize. II. The location of the controlling element alleles. Genetics 60:507–524.Google Scholar
  12. 12.
    Roman, H. (1947) Mitotic nondisjunction in the case of interchanges involving the B-type chromosome in maize. Genetics 32:391–409.Google Scholar
  13. 13.
    Van der Walt, W.J. (1967) Paramutation of R alleles of diverse geographic origin. Ph.D. Thesis, University of Wisconsin, Madison.Google Scholar
  14. 14.
    Wessler, S., and M.J. Varagona (1985) Molecular basis of mutations at the waxy locus of maize: Correlation with the fine structure map. Proc. Natl. Acad. Sci., USA 82:4177–4181.PubMedCrossRefGoogle Scholar
  15. 15.
    Williams, W.M., K.V. Satyanarayana, and J.L. Kermicle (1984) R-stippled maize as a transposable element system. Genetics 107:477–488.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Jerry L. Kermicle
    • 1
  1. 1.Laboratory of GeneticsUniversity of WisconsinMadisonUSA

Personalised recommendations