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Structure and DNA Modification of Endogenous Mu Elements

  • Vicki L. Chandler
  • Luther E. Talbert
  • Laura Mann
  • Catherine Faber
Part of the Basic Life Sciences book series (BLSC, volume 47)

Summary

The high mutation rate of Mutator stocks of maize is the result of transposition of Mu elements. Most standard stocks of maize contain sequences homologous to Mu elements, yet they show no genetic activity characteristic of Mu transposition. We have determined the sequence, structure, and genomic organization of several Mu-homologous elements from non-Mutator lines. Our results suggest that these elements transposed into their current genomic positions. We discuss the relationship of these endogenous Mu elements to active elements and the role DNA modification may play in their apparent inactivity.

Keywords

Transposable Element High Mutation Rate Terminal Inverted Repeat Maize Line Mutator Stock 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Alleman, M., and M. Freeling (1986) The Mutransposable element of maize: Evidence for transposition and copy number regulation during development. Genetics 112:107–119.PubMedGoogle Scholar
  2. 2.
    Bennetzen, J.L., J. Swanson, W.C. Taylor, and M. Freeling (1984) DNA insertion in the first intron of maize Adh1 affects message levels: Cloning of progenitor and mutant Adh1 alleles. Proc. Natl. Acad. Sci., USA 81:4125–4128.PubMedCrossRefGoogle Scholar
  3. 3.
    Bennetzen, J. (1987) Covalent DNA modification and the regulation of Mutator element transposition in maize. Molec. Gen. Genet. 208:45–51.CrossRefGoogle Scholar
  4. 4.
    Bennetzen, J.L., R.P. Fracasso, D.W. Morris, D.S. Robertson, and M.J. Skogen-Hagenson (1987) Concomitant regulation of Mu1 transposition and Mutator activity in maize. Molec. Gen. Genet. 208:57–62.CrossRefGoogle Scholar
  5. 5.
    Burr, B., and F. Burr (1981) Transposable elements and genetic instabilities in crop plants. Stadler Genet. Symp. 13:115–128.Google Scholar
  6. 6.
    Chandler, V., and V. Walbot (1986) DNA modification of a maize transposable element correlates with loss of activity. Proc. Natl. Acad. Sci., USA 83:1767–1771.PubMedCrossRefGoogle Scholar
  7. 7.
    Chandler, V., C. Rivin, and V. Walbot (1986) Stable non-Mutator stocks of maize have sequences homologous to the Mu1 transposable element. Genetics 114:1007–1021.PubMedGoogle Scholar
  8. 8.
    Chen, C.-H., K. Oishi, B. Kloeckener-Gruissem, and M. Freeling (1987) Organ-specific expression of maize Adh1 is altered after a Mu transposon insertion. Genetics 116:469–477.PubMedGoogle Scholar
  9. 9.
    Chomet, P.S., S. Wessler, and S. Dellaporta (1987) Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J. 6:295–302.PubMedGoogle Scholar
  10. 10.
    Döring, H.-P., and P. Starlinger (1984) Barbara McClintock’s controlling elements: Now at the DNA level. Cell 39:253–259.PubMedCrossRefGoogle Scholar
  11. 11.
    Lillis, M., and M. Freeling (1986) Mu transposons in maize. Trends in Genet. 2:183–187.CrossRefGoogle Scholar
  12. 12.
    McClintock, B. (1951) Chromosome organization and genic expression. Cold Spring Harbor Symposia on Quantitative Biology 16:13–47.PubMedCrossRefGoogle Scholar
  13. 13.
    McClintock, B. (1978) Mechanisms that rapidly reorganize the genome. Stadler Genet. Symp. 10:25–48.Google Scholar
  14. 14.
    McClintock, B. (1984) The significance of responses of the genome to challenge. Science 226:792–801.PubMedCrossRefGoogle Scholar
  15. 15.
    McLaughlin, M., and V. Walbot (1987) Cloning of a mutable bz2 allele of maize by transposon tagging and differential hybridization. Genetics 117:771–776.PubMedGoogle Scholar
  16. 16.
    O’Reilly, C., N.S. Shepherd, A. Pereira, Zs. Schwarz-Sommer, I. Bertram, D.S. Robertson, P.A. Peterson, and H. Saedler (1985) Molecular cloning of the al locus of Zea mays using the transposable elements En and Mu1. EMBO J. 4:877–882.PubMedGoogle Scholar
  17. 17.
    Roberts, D., B.C. Hoopes, W.R. McClure, and N. Kleckner (1985) IS 10 transposition is regulated by DNA adenosine methylation. Cell 43:117–130.PubMedCrossRefGoogle Scholar
  18. 18.
    Robertson, D.S. (1978) Characterization of a Mutator system in maize. Mutat. Res. 51:21–28.CrossRefGoogle Scholar
  19. 19.
    Robertson, D.S., and P.N. Mascia (1981) Tests of 4 controlling-element systems for mutator activity and their interaction with Mumutator. Mutat. Res. 84:283–289.CrossRefGoogle Scholar
  20. 20.
    Robertson, D.S. (1983) A possible dose dependent inactivation of Mutator (Mu) in maize. Molec. Gen. Genet. 191:86–90.CrossRefGoogle Scholar
  21. 21.
    Robertson, D.S. (1986) Genetic studies on the loss of Mumutator activity in maize. Genetics 113:765–773.PubMedGoogle Scholar
  22. 22.
    Schwartz, D., and E. Dennis (1986) Transposase activity of the Ac controlling element in maize is regulated by its degree of methylation. Molec. Gen. Genet. 205:476–482.CrossRefGoogle Scholar
  23. 23.
    Talbert, L.E., D. Turks, C. Faber, L. Mann, K. Sylvester, F. Raymond, and V.L. Chandler (1987) Molecular genetic analysis of sequences homologous to Mutator transposable elements in non-Mutator maize stocks. In Plant Molecular Biology, D. von Wettstein and N.-H. Chua, eds. Plenum Press, New York, pp. 181–189.Google Scholar
  24. 24.
    Taylor, L., V. Chandler, and V. Walbot (1986) Insertion of 1.4 kb and 1.7 kb Muelements into the bronze-1 gene of Zea mays L. Maydica 31:31–45.Google Scholar
  25. 25.
    Taylor, L., and V. Walbot (1987) Isolation and characterization of a 1.7 kb transposable element from a Mutator line of maize. Genetics 117:297–307.PubMedGoogle Scholar
  26. 26.
    Walbot, V. (1986) Inheritance of mutator activity in Zea mays as assayed by somatic instability of the bz2-mul allele. Genetics 114:1293–1312.PubMedGoogle Scholar
  27. 27.
    Walbot, V., and C. Warren (1988) Regulation of Mu element copy number in maize lines with an active or inactive Mutator transposable element system. Molec. Gen. Genet. 211:27–34.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Vicki L. Chandler
    • 1
  • Luther E. Talbert
    • 1
  • Laura Mann
    • 1
  • Catherine Faber
    • 1
  1. 1.Institute of Molecular BiologyUniversity of OregonEugeneUSA

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