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A New Mu Element from a Robertson’s Mutator Line

  • Karen K. Oishi
  • Michael Freeling
Part of the Basic Life Sciences book series (BLSC, volume 47)

Abstract

Robertson (8) described a maize line that, when self-pollinated, generated a 50-fold higher frequency of recessive mutants than expected spontaneously. Approximately 40% of these mutations were unstable genetically, a characteristic of transposon-induced mutants. The first insertional mutagen from a Robertson’s Mutator line, Mu1, was identified and cloned from an unstable mutant allele of Adh1 (3,9). Nine bp of genomic intron-1 DNA was duplicated upon insertion. A complete sequence analysis (2) of the Mu1 element determined that the 1,367-bp element contained 215-bp terminal repeats and 100-bp internal direct repeats. All Mutator lines were found to contain 10–70 copies of Mu1-homologous sequences (1,3). Zero-2 copies of Mu1 exist in non-Mutator lines (3,6). There are one larger and three smaller Mu1-related elements in Mutator lines (10). The largest-sized Mu1-family element described, Mu2 (also called Mu1.7), has a few hundred additional base pairs of internal DNA as compared to Mu1 (10).

Keywords

Mutator Line Terminal Inverted Repeat Maize Line Adh1 Gene Terminal Inverted Repeat Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Karen K. Oishi
    • 2
  • Michael Freeling
    • 1
  1. 1.Department of GeneticsUniversity of CaliforniaBerkeley
  2. 2.Molecular and Cellular Biology Department and Plant Sciences DepartmentUniversity of ArizonaTucsonUSA

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